| VEGF真核表达载体构建及其在骨髓间充质干细胞中的表达 |
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| 引用本文： | 高峰,王红兵,俞世强,蔡振杰. VEGF真核表达载体构建及其在骨髓间充质干细胞中的表达[J]. 心脏杂志, 2006, 18(2): 135-137. DOI: 10.13191/j.chj.2006.02.21.gaof.006 |
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| 作者姓名： | 高峰 王红兵 俞世强 蔡振杰 |
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| 作者单位： | 第四军医大学西京医院心血管外科研究所,陕西,西安,710032 |
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| 摘 要： | 目的构建血管内皮生长因子（vascu lar endothelial growth factor,VEGF）真核表达载体,并研究其在骨髓间充质干细胞（m esenchym al stem cells,MSCs）中的表达情况。方法运用DNA重组技术,构建VEGF真核表达载体pcDNA3.1（-）/hVEGF165,将载体转染大鼠MSCs,RT-PCR,ELISA及免疫细胞化学等方法检测VEGF在大鼠MSCs中的表达情况,MTT法检测转染后细胞上清液中VEGF的生物活性。结果成功构建VEGF真核表达载体pcDNA3.1（-）/hVEGF165,其转染后的大鼠MSCs中VEGF表达水平明显增高,转染后细胞培养上清液具有促使内皮细胞增殖的生物活性。结论VEGF真核表达载体转染大鼠MSCs后能有效增加VEGF表达,并表现出较强的促内皮细胞增殖作用。
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| 关 键 词： | 血管内皮细胞生长因子 真核表达载体 骨髓间充质干细胞 |
| 文章编号： | 1009-7236(2006)02-135-03 |
| 修稿时间： | 2005-09-20 |
Construction of VEGF eukaryotic expression vector and its expression in mesenchymal stem cells |
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| GAO Feng,WANG Hong-bing,YU Shi-qiang,CAI Zhen-jie. Construction of VEGF eukaryotic expression vector and its expression in mesenchymal stem cells[J]. Chinese Heart Journal, 2006, 18(2): 135-137. DOI: 10.13191/j.chj.2006.02.21.gaof.006 |
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| Authors: | GAO Feng WANG Hong-bing YU Shi-qiang CAI Zhen-jie |
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| Abstract: | AIM To construct eukaryotic expression vector of human vascular endothelial growth factor(VEGF) gene and to investigate its transfection and expression in rat mesenchymal stem cells(MSCs).METHODS After pcDNA3.1(-)/hVEGF165 vector was successfully constructed,rat MSCs were transfected with jet-PEI.RT-PCR,ELISA and immuocytochemical methods were used to detect the expression of VEGF and MTT was used to detect the biological activity. RESULTS VEGF mRNA and protein in the transfected group significantly increased and obvious biological activity was observed in stimulating proliferation of endothelial cells.CONCLUSION pcDNA3.1(-)/hVEGF165 vector can effectively transfect rat MSCs.The transfected rat MSCs significantly increase VEGF mRNA and protein expression,and have relatively strong biological activity. |
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| Keywords: | vascular endothelial growth factor eukaryotic expression vector mesenchymal stem cells |
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