Evidence for in vivo incorporation of D-lactate into peptidoglycan precursors of vancomycin-resistant enterococci. |
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| Authors: | M Arthur C Molinas T D Bugg G D Wright C T Walsh P Courvalin |
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| Affiliation: | Unité des Agents Antibactériens, Institut Pasteur, Paris, France. |
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| Abstract: | The VanA ligase encoded by the vancomycin resistance plasmid pIP816 of Enterococcus faecium BM4147 condenses D-alanine with various D-2-hydroxy and D-2-amino acids in vitro. D-Lactate added to the culture medium restored the vancomycin resistance of a strain that does not produce the VanH dehydrogenase and therefore appears to be a substrate of VanA in vivo. |
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