| CHST13抑制肝癌细胞侵袭能力的研究 |
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| 引用本文： | 李艳平,刘有训,马红叶,王宁,张旭. CHST13抑制肝癌细胞侵袭能力的研究[J]. 国际肿瘤学杂志, 2014, 41(4): 314-318 |
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| 作者姓名： | 李艳平 刘有训 马红叶 王宁 张旭 |
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| 作者单位： | 李艳平 (116044,大连医科大学检验医学院)； 刘有训 (116044,大连医科大学检验医学院)； 马红叶 (116044,大连医科大学检验医学院)； 王宁 (116044,大连医科大学检验医学院)； 张旭 (116044,大连医科大学检验医学院)； |
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| 摘 要： | 目的 通过研究磺基转移酶CHST13在人肝癌高转移细胞株MHCC97-H和人肝癌低转移细胞株MHCC97-L中的差异表达,明确其与肝癌转移的相关性,从而证实肝癌转移诊断及抗肿瘤治疗新靶点.方法 采用实时PCR、Western blot分析磺基转移酶CHST13在人肝癌高、低转移细胞株的差异表达,通过RNA干扰技术干预CHST13基因,检测干扰前后MHCC97-L细胞的体外侵袭力及体内成瘤性.结果 CHST13在人肝癌高、低转移细胞株中表达具有明显差异;下调MHCC97-L细胞中CHST13基因表达后,穿过基底膜的细胞数量[（30±3）个]明显多于未处理组[（14±2）个],体外侵袭力显著增强（t=2.8,P=0.005）;相对于正常细胞[（0.5±0.06）g],干扰后细胞的肿瘤平均重量[（0.9±0.10）g]明显增大,体内成瘤性明显提高（t=2.5,P=0.01）.结论 磺基转移酶CHST13与肝癌细胞的侵袭、成瘤性密切相关,其有望成为肝癌临床治疗的新靶点.
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| 关 键 词： | 磺基转移酶类 癌,肝细胞 肿瘤转移 |
CHST13 inhibits the invasive property of human hepatocellular carcinoma cells |
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| Li Yanping,Liu Youxun,Ma Hongye,Wang Ning,Zhang Xu. CHST13 inhibits the invasive property of human hepatocellular carcinoma cells[J]. Journal of International Oncology, 2014, 41(4): 314-318 |
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| Authors: | Li Yanping Liu Youxun Ma Hongye Wang Ning Zhang Xu |
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| Affiliation: | . College of Medical Laboratory, Dalian Medical University, Dalian 116044, China |
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| Abstract: | Objective To clarify the association between chondroitin-4-O-sulfotransferase-3 （CHST13） and hepatoma metastasis by the research of differential expression of CHST13 in MHCC97-H and MHCC97-L human hepatocarcinoma cell lines, which have high and low metastatic potential, respectively, and to confirm novel target of hepatoma metastasis and anti-tumor therapy. Methods The differential expressions of CHST13 in MHCC97-H and MHCC97-L human hepatocarcinoma cell lines were analyzed by real-time PCR, Western blot. CHST13 was silenced using RNA interference approach to detect the invasive ability in vitro and tumorigenicity in vivo in MHCC97-L cells. Results The expression of CHST13 was different in MHCC97-L cells, as compared to those in MHCC97-H cells. Knockdown of CHST13 expression enhanced MHCC97-L ceils invasion and tumorigenicity both in vitro （t = 2.8, P = 0. 005 ） and in vivo （ t = 2.5, P = 0.01 ）. The quantity of cells which crossed basement membrane increased [ （30 ± 3 ） ： （ 14 ± 2） ], and the average weight of tumor increased [ （0.9 ± 0. 10） g ： （0.5 ± 0.06 ） g ]. Conclusion The differential expressions of CHST13 in human hepatocarcinoma cell lines correlate with tumor invasion and tumorigenicity, and it is expected to be a novel target for the treatment of hepatocellular carcinoma. |
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| Keywords: | Sulfotransferases Carcinoma, hepatocellular Neoplasm metastasis |
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