| 膝关节软骨中基质金属蛋白酶13及其组织抑制剂1的表达 |
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| 引用本文： | 杨立群,张国彬,习瑾昆,田发明. 膝关节软骨中基质金属蛋白酶13及其组织抑制剂1的表达[J]. 中国组织工程研究, 2015, 19(15): 2310-2314. DOI: 10.3969/j.issn.2095-4344.2015.15.003 |
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| 作者姓名： | 杨立群 张国彬 习瑾昆 田发明 |
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| 作者单位： | 华北理工大学，基础医学院，研究生学院，医学实验研究中心，河北省唐山市 063000 |
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| 基金项目： | 河北联合大学博士科研启动基金资助项目；唐山市老年医学重点实验室资助项目(14140221B) |
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| 摘 要： | 背景：基质金属蛋白酶组织抑制剂1是与基质金属蛋白酶13相对应的拮抗剂，两者间表达水平和功能活性的平衡对细胞外基质的代谢状态起着重要作用，但在DH豚鼠骨关节炎发生发展过程中，两者表达水平，尤其两者表达水平比值的变化尚不明确。目的：探讨不同月龄DH豚鼠关节软骨中基质金属蛋白酶13、基质金属蛋白酶组织抑制剂1表达比值的变化及其与DH豚鼠增龄性原发骨性关节炎发病过程中软骨退变程度的关系。方法：选取2，4，8，12月龄雌性DH各6只，取膝关节观察关节软骨的大体形态后常规脱钙、包埋、制作石蜡切片，用于VG染色行组织学观察，采用Mankin评分系统定量分析关节软骨退变情况，采用免疫组织化学方法检测膝关节软骨中基质金属蛋白酶13、基质金属蛋白酶组织抑制剂1表达情况，应用 Image pro-Plus 6.0软件对免疫组织化学阳性蛋白表达情况进行积分吸光度计算。线性回归分析判断Mankin评分与基质金属蛋白酶13/基质金属蛋白酶组织抑制剂1比值的相关性。结果与结论：DH豚鼠2月龄膝关节软骨无关节炎表现，4 月龄出现轻度软骨退变，并随月龄增加进行性加重，Mankin评分随月龄增加逐渐增高，各组之间的差异均有显著性意义(P < 0.05)。基质金属蛋白酶13、基质金属蛋白酶组织抑制剂1的表达均随月龄进行性增加，两者比值与Mankin评分呈正相关性(P < 0.05)。结果提示DH豚鼠4月龄出现膝关节软骨退行性变化，随月龄增加而进行性加重，其病理改变与基质金属蛋白酶13/基质金属蛋白酶组织抑制剂1表达失衡有关。中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程全文链接：
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| 关 键 词： | 组织构建 软骨组织工程 基质金属蛋白酶13 DH豚鼠 骨性关节炎 基质金属蛋白酶组织抑制剂1 |
Expression of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase 1 in knee articular cartilage |
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| Yang Li-qun,Zhang Guo-bin,Xi Jin-kun,Tian Fa-ming. Expression of matrix metalloproteinase-3 and tissue inhibitor of metalloproteinase 1 in knee articular cartilage[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(15): 2310-2314. DOI: 10.3969/j.issn.2095-4344.2015.15.003 |
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| Authors: | Yang Li-qun Zhang Guo-bin Xi Jin-kun Tian Fa-ming |
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| Affiliation: | Basic Medicine School, 2raduate School, Medical Research Center, North China University of Science and Technology, Tangshan 063000, Hebei Province, China |
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| Abstract: | BACKGROUND:Tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) is the corresponding antagonist of matrix metalloproteinase 13 (MMP-13), and their balance between expression and functional activity exerts an important role in the metabolic state of the extracellular matrix. During the development of osteoarthritis, however, TIMP-1 and MMP-13 expressions and their expression ratio show unclear changes in DH guinea pigs.OBJECTIVE:To explore the expression levels of MMP-13 and TIMP-1 in DH guinea pigs with different ages, and to analyze the relationship between the ratio of MMP-13 to TIMP-1 and the age-dependent degenerative changes in the articular cartilage.METHODS:Twenty-four female Dunkin Hartley guinea pigs were sacrificed at age of 2, 4, 8, 12 months separately, with six animals at each time point. The knee joints were collected and gross visual appearance of the articular cartilage was observed, then were decalcified and prepared for paraffin sections. VG staining and Mankin score were used to analyze the histological changes. Immunohistochemistry was conducted to assess the expression levels of MMP-13 and TIMP-1 in the cartilage. Integrated absorbance values were used as the quantitive analysis calculated by Image pro-Plus 6.0. Linear regression analysis was done to analysis the relationship between Mankin score and the ratio of MMP-13/TIMP-1.RESULTS AND CONCLUSION:Normal appearance in the articular cartilage was observed in 2-month-old DH guinea pigs, while degenerative changes in the articular cartilage were shown in 4-month-old animals, which became severer with age. Significant difference was found in Mankin score between any two groups (P < 0.05). The ratio of MMP-13 to TIMP-1 increased with age, and the ratio was positively correlated to the Mankin score (P < 0.05). Age-related articular cartilage degeneration occurred in Dunkin Hartley guinea pigs at 4 months of age, and devoloped with age, which is related with the imbanlance of the expression ratio of MMP-13 to TIMP-1. |
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| Keywords: | Osteoarthritis Matrix Metalloproteinases Guinea Pigs |
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