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| 灯盏花素干预糖尿病模型大鼠睾丸组织增殖细胞核抗原和c-fos的表达 | |
| 引用本文： | 龙玲莉,郑淑慧,李宇彬. 灯盏花素干预糖尿病模型大鼠睾丸组织增殖细胞核抗原和c-fos的表达[J]. 中国组织工程研究, 2015, 19(18): 2917-2922. DOI: 10.3969/j.issn.2095-4344.2015.18.023 |
| 作者姓名： | 龙玲莉 郑淑慧 李宇彬 |
| 作者单位： | 中山大学附属第一医院，1转化中心实验室，2生殖医学中心，广东省广州市 510080 |
| 基金项目： | 国家自然科学基金(81100470) |
| 摘 要： | 背景：有研究表明灯盏花素可影响2型糖尿病大鼠的生殖能力，但其作用机制少有报道。目的：探讨灯盏花素对2型糖尿病大鼠睾丸增殖细胞核抗原和原癌基因c-fos表达的影响作用。方法：选取36只健康雄性大鼠随机分为对照组、模型组和灯盏花素组各12只。模型组和灯盏花素组采用链脲佐菌素连续腹腔注射建立2型糖尿病大鼠模型，大鼠血糖监测达到16.7 mmol/L作为建模标准，对照组大鼠予以同等容积的柠檬酸缓冲液单次腹腔注射。灯盏花素组采用灯盏花素注射液10 mg/(kg•d)连续4周腹腔注射，其他2组在相同时间注入等量生理盐水。结果与结论：干预4周后，血清睾酮检测、免疫组织化学染色和PCR检测结果显示，血清睾酮水平、增殖细胞核抗原、c-Fos蛋白及mRNA表达：对照组>灯盏花素组>模型组(P < 0.05)；血糖水平：对照组<灯盏花素组<模型组(P < 0.05)。结果证实，灯盏花素可以通过增强增殖细胞核抗原和c-fos的表达，保护2型糖尿病大鼠的生殖功能。中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程全文链接： |
| 关 键 词： | 动物模型 基因病毒载体及相关因子模型 灯盏花素 2型糖尿病 睾丸增殖细胞核抗原 睾丸增殖细胞核抗原转录基因 c-Fos蛋白 原癌基因 大鼠 睾酮 链脲佐菌素 生殖功能 国家自然科学基金 |
| 收稿时间： | 2015-02-15 |
Breviscapine effects on the expression of proliferating cell nuclear antigen and c-fos in the testis of diabetic rat models |
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| Long Ling-li,Zheng Shu-hui,Li Yu-bin. Breviscapine effects on the expression of proliferating cell nuclear antigen and c-fos in the testis of diabetic rat models[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2917-2922. DOI: 10.3969/j.issn.2095-4344.2015.18.023 | |
| Authors: | Long Ling-li Zheng Shu-hui Li Yu-bin |
| Affiliation: | 1Transfer Center Laboratory, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; 2Center for Reproductive Medicine, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China |
| Abstract: | BACKGROUND: Breviscapine has been shown to impact the reproductive capacity in rats with type 2 diabetes mellitus, but few reports concerned its mechanism of action. OBJECTIVE: To study the effects of breviscapine on proliferating cell nuclear antigen and proto-oncogene c-fos expression in testis of type 2 diabetes mellitus rats. METHODS: Totally 36 healthy male rats were randomly divided into control group, model group and breviscapine group with 12 rats in each group. In the model group and breviscapine group, rat models of type 2 diabetes mellitus were established by continuous intraperitoneal injection of streptozotocin. Blood glucose reaching16.7 mmol/L in rats was considered as the standard of model induction. In the control group, rats were given an equal volume of citrate buffer solution by single intraperitoneal injection. In the breviscapine group, rats were administered breviscapine 10 mg/(kg•d) for 4 consecutive weeks by intraperitoneal injection. Rats in the other two groups were injected with an equal volume of physiological saline at the same time point. RESULTS AND CONCLUSION: After 4 weeks of intervention, serum testosterone testing, immunohistochemistry and PCR results showed that serum testosterone levels, proliferating cell nuclear antigen, c-Fos protein and mRNA expression: control group > breviscapine group > model group (P < 0.05); blood glucose concentration: the control group < breviscapine group < model group (P < 0.05). Results confirmed that breviscapine can protect the reproductive function in type 2 diabetes mellitus rats by enhancing the expression of proliferating cell nuclear antigen and c-fos. |
| Keywords: | Tissue Engineering Diabetes Mellitus Proto-Oncogenes Testosterone |
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