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Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients
Authors:Heo Eun-jeong  Park Jin-ho  Koo Ja-ryong  Park Man-suk  Park Mi-yeoun  Dumler J Stephen  Chae Joon-seok
Affiliation:Department of Veterinary Internal Medicine and Clinical Pathology, College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea.
Abstract:Sera from 491 Korean patients with acute febrile diseases were tested for Ehrlichia chaffeensis and Anaplasma phagocytophila antibodies by indirect immunofluorescence assay (IFA), Western blotting, and TaqMan real-time PCR. Overall, 0.4% of sera reacted with E. chaffeensis, and 1.8% reacted with A. phagocytophila in IFAs. This is the first report of detection of antibodies to A. phagocytophila and E. chaffeensis in Korea and suggests the presence of A. phagocytophila and E. chaffeensis or antigenically similar species.
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