Group B Arbovirus Structural and Nonstructural Antigens II. Purification of Saint Louis Encephalitis Virus Intracellular Antigens |
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| Authors: | Ateef A. Qureshi and Dennis W. Trent |
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| Affiliation: | Department of Microbiology, The University of Texas Medical School at San Antonio, San Antonio, Texas 78284 |
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| Abstract: | Three serologically distinct antigens were identified in homogenates of Saint Louis encephalitis (SLE) virus-infected cells after Brij-58 solubilization and diethylaminoethyl (DEAE)-cellulose chromatography. These antigens were designated as antigen I, II, and III. Immunodiffusion analyses showed that all antigen peaks which eluted from the DEAE-cellulose column contained the intracellular major envelope protein, antigen I. This protein was the only viral antigen which eluted at 0.125 M KCl in DEAE-cellulose column peak C. Optical density peak D, which eluted at 0.2 M KCl, contained both antigens I and III. Antigen III was purified from this column fraction approximately 200-fold by organic solvent extraction and chromatography on hydroxylapatite and Sepharose 6B. Purified antigen III had a molecular weight of 80,000 to 85,000 and contained only one antigenic determinant as judged by immunodiffusion analysis. |
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