| 两色金鸡菊醇提物对2型糖尿病小鼠肠道菌群的影响 |
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| 引用本文： | 张凯楠,龙梅,冉新建,阳迎,仝磊,李清晨,熊健会,毛新民,陶亮,李琳琳. 两色金鸡菊醇提物对2型糖尿病小鼠肠道菌群的影响[J]. 实验动物与比较医学, 2015, 23(6): 628-633 |
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| 作者姓名： | 张凯楠 龙梅 冉新建 阳迎 仝磊 李清晨 熊健会 毛新民 陶亮 李琳琳 |
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| 作者单位： | 新疆医科大学,新疆医科大学基础学院,新疆医科大学基础学院,新疆医科大学工程技术学院,新疆医科大学工程技术学院,新疆乌鲁木齐市新医路393号,新疆医科大学药学院,新疆医科大学药学院,新疆医科大学 |
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| 基金项目： | 自治区科技计划项目课题：新疆创新药物临床前药效学评价技术平台建设（201233150）；SRTP（20140760002） |
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| 摘 要： | 【摘要】目的研究2型糖尿病动物模型与正常ICR小鼠肠道菌群的差异和两色金鸡菊醇提物对小鼠肠道菌群的影响，探讨两色金鸡菊醇提物，肠道菌群和2型糖尿病(T2DM)之间的可能联系。方法收集模型组（A）,两色金鸡菊醇提物高剂量组1.8g/kg（B）, 两色金鸡菊醇提物中剂量组1.2g/kg(C), 二甲双胍对照组0.2g/kg（D）,空白对照组(E)粪便样本，采用16S rDNA实时荧光定量PCR技术检测粪便样本中球形梭菌与多形拟杆菌的水平；运用Pearson分析方法，对目标菌属水平与各组小鼠的空腹血糖( FBG)做相关性分析。结果1.与正常组比较，模型组中球形梭菌与多形拟杆菌水平均增高(p=0.017，p=0.002)。2.两色金鸡菊醇提物高剂量组与模型组相比，球形梭菌与多形拟杆菌的水平下降，且有统计学差异（2周:p=0.027,p=0.006;4周：p=0.007，p=0.012）。3.球形梭菌水平、多形拟杆菌水平均与FBG水平呈正相关。结论两色金鸡菊醇提物对2型糖尿病小鼠肠道菌群存在着一定的影响且与其血糖水平存在相关性。
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| 关 键 词： | 球形梭菌；多形拟杆菌；2型糖尿病；荧光定量PCR |
| 收稿时间： | 2015-06-16 |
| 修稿时间： | 2015-07-15 |
Effect of alcohol extract of Coreopsis tinctoria Nutt on intestinal flora in type 2 diabetic mice |
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| ZHANG Kai-nan,LONG Mei,RAN Xin-jian,YANG Ying,TONG Lei,LI Qing-cheng,XIONG Jian-hui,MAO Xin-min,TAO Liang and LI Lin-lin. Effect of alcohol extract of Coreopsis tinctoria Nutt on intestinal flora in type 2 diabetic mice[J]. Laboratory Animal and Comparative Medicine, 2015, 23(6): 628-633 |
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| Authors: | ZHANG Kai-nan LONG Mei RAN Xin-jian YANG Ying TONG Lei LI Qing-cheng XIONG Jian-hui MAO Xin-min TAO Liang LI Lin-lin |
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| Affiliation: | The Clinical Medical School, Xinjiang Medical University, Urumchi 830054, China;Central Laboratory of Functional Activities, Xinjiang Medical University, Urumchi 830054, China;Central Laboratory of Functional Activities, Xinjiang Medical University, Urumchi 830054, China;The Clinical Medical School, Xinjiang Medical University, Urumchi 830054, China;The Clinical Medical School, Xinjiang Medical University, Urumchi 830054, China;The Clinical Medical School, Xinjiang Medical University, Urumchi 830054, China;Department of Pharmacology, Xinjiang Medical University, Urumchi 830054, China;The School of Traditional Chinese Medicine, Xinjiang Medical University, Urumchi 830054, China;Department of Pharmacology, Xinjiang Medical University, Urumchi 830054, China;Department of Pharmacology, Xinjiang Medical University, Urumchi 830054, China |
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| Abstract: | Objective To study the differences in intestinal flora of normal and type 2 diabetic mice, the effect of alcoholic extract of Coreopsis tinctoria Nutt on mouse intestinal flora, and explore the possible relationship between alcoholic extract of Coreopsis tinctoria Nutt, intestinal flora and type 2 diabetes mellitus (T2DM) in mice. Methods Stool samples were collected from the normal control group (A), high dose (1.8 g/kg) (B) and moderate dose (1.2 g/kg) (C) alcoholic extract of Coreopsis tinctoria Nutt model groups, metformin (0.2 g/kg) treatment group (D) and blank control (E) group. 16S rDNA real-time quantitative PCR assay was used to determine the levels of Clostridium coccoides and Bacteroides thetaiotaomicron in the stool samples. Pearson analysis method was used to analyze the correlation between the levels of target bacterial species and the fasting blood glucose (FBG) in the mice. Results 1. Compared with the normal control group, the levels of Clostridium coccoides and Bacteroides thetaiotaomicron in the T2DM model group were significantly lowered (P=0.017, P=0.002). 2. Compared with the model group, the levels of Clostridium coccoides and Bacteroides thetaiotaomicron of the high dose Coreopsis tinctoria Nutt alcohol extract group were significantly different (2 weeks:P=0.027, P=0.006; 4 weeks: P=0.007, P=0.012). 3. The levels of Clostridium coccoides and Bacteroides thetaiotaomicron were positively correlated with the FBG level in the mice. Conclusions The alcohol extract of Coreopsis tinctoria Nutt has certain effect on the intestinal flora in type 2 diabetic mice and there is certain correlation between the effect of alcohol extract of Coreopsis tinctoria Nutt and their blood glucose level. |
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| Keywords: | Clostridium coccoides Bacteroides thetaiotaomicron Type 2 diabetes mellitus Real-time PCR |
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