| 氢化可的松致肾虚证小鼠模型的建立及相关指标的评价 |
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| 引用本文： | 戴冰,张嘉妮,杨梦琳,李玉星,肖子曾,杨磊,韦强. 氢化可的松致肾虚证小鼠模型的建立及相关指标的评价[J]. 实验动物与比较医学, 2017, 25(1): 70-73 |
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| 作者姓名： | 戴冰 张嘉妮 杨梦琳 李玉星 肖子曾 杨磊 韦强 |
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| 作者单位： | 湖南中医药大学第一附属医院, 长沙 410007;湖南中医药大学, 长沙 410208;湖南中医药大学, 长沙 410208;湖南中医药大学, 长沙 410208;湖南中医药大学, 长沙 410208;湖南中医药大学第一附属医院, 长沙 410007;湖南中医药大学第一附属医院, 长沙 410007 |
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| 基金项目： | 湖南省教育厅重点项目（编号：15A140）；湖南中医药大学研究生科研创新课题（编号：2016CX16）。 |
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| 摘 要： | 目的 建立氢化可的松致肾阳虚、肾阴虚小鼠模型，并探讨其相关评价指标。方法 采用氢化可的松灌胃建立肾阳虚及肾阴虚小鼠模型；观察肾阳虚、肾阴虚模型小鼠生存状态，测定各组模型小鼠体重变化；采用放射免疫法检测HPA轴中促肾上腺皮质激素（ACTH）、皮质醇（Cor.）、HPT轴中甲状腺激素（TSH）、三碘甲腺原氨酸（T3）、甲状腺素（T4）、HPG轴中促卵泡素（FSH）、雌二醇（E2）、睾酮（T）含量。结果 肾阳虚、肾阴虚模型小鼠体重均下降，肾阳虚模型小鼠血清ACTH、Cor.、TSH、T3、T4水平降低，血清FSH、E2、T水平升高，且差异均有显著性（P<0.01）；而肾阴虚模型则反之。结论 肾虚小鼠内分泌系统中ACTH、Cor.、TSH、T3、T4、FSH、E2、T各激素水平均发生了改变，其中Cor.可作为评价糖皮质激素法建立肾虚模型的重要指标。
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| 关 键 词： | 肾阳虚 肾阴虚 评价指标 |
| 收稿时间： | 2016-06-13 |
Establishment of a mouse model of kidney deficiency induced by oral administration of hydrocortisone and evaluation of related factors |
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| DAI Bing,ZHANG Jia-ni,YANG Meng-lin,LI Yu-xing,XIAO Zi-zeng,YANG Lei and WEI Qiang. Establishment of a mouse model of kidney deficiency induced by oral administration of hydrocortisone and evaluation of related factors[J]. Laboratory Animal and Comparative Medicine, 2017, 25(1): 70-73 |
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| Authors: | DAI Bing ZHANG Jia-ni YANG Meng-lin LI Yu-xing XIAO Zi-zeng YANG Lei WEI Qiang |
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| Affiliation: | The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China;Hunan University of Chinese Medicine, Changsha 410208;Hunan University of Chinese Medicine, Changsha 410208;Hunan University of Chinese Medicine, Changsha 410208;Hunan University of Chinese Medicine, Changsha 410208;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410007, China |
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| Abstract: | Objective To establish a mouse model of kidney-yin and kidney-yang deficiency after oral administration of hydrocortisone, and to explore the related evaluation factors.Methods The model was established by oral administration of hydrocortisone to induce kidney-yin and kidney-yang deficiency in mice. The survival and body weight of the mice were observed. The serum content of adrenal cortical hormone (ACTH), cortisol (Cor.) in the hypothalamic-pituitary-adrenal (HPA) axis, and thyroid stimulating hormone (TSH), triiodothyronine (T3), thyrox (T4) in the hypothalamic-pituitary-thyroid (HPT) axis, follicle-stimulating hormone (FSH), estradiol (E2), testosterone (T) in the hypothalamic-pituitary-gonadal (HPG) axis were determined by radioimmunoassay.Results The body weight of kidney-yin and kidney-yang mice were decreased, the serum ACTH, Cor, TSH, T3, T4 contents were decreased, the serum FSH, E2, T contents were increased in the kidney-yang deficiency model mce (P<0.01), and those parameters in the kidney-yin deficiency model mice were changed in opposite direction. Conclusions It is found that the hormone levels of ACTH, Cor, TSH, T3, T4, FSH, E2 and T in kidney deficiency mice are changed, and cortisol can be used as an important index to evaluate the model of kidney deficiency induced by glucocorticoid. |
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| Keywords: | Kidney-yang deficiency Kidney-yin deficiency Evaluating factors Mouse models |
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