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| 富血小板血浆对犬骨髓间充质细胞矿化能力的影响 | |
| 引用本文： | 潘永海,李宁毅,邱建忠,金晓明,陈立强,刘相萍,隋爱华. 富血小板血浆对犬骨髓间充质细胞矿化能力的影响[J]. 口腔医学, 2007, 27(5): 232-235 |
| 作者姓名： | 潘永海 李宁毅 邱建忠 金晓明 陈立强 刘相萍 隋爱华 |
| 作者单位： | 青岛大学医学院二附院,青岛市中心医院口腔颌面外科,青岛,266042;青岛大学医学院附属医院口腔颌面外科,青岛,266003;青岛大学医学院附属市立医院口腔颌面外科,青岛,266031;青岛大学医学院附属医院分子生物实验室,青岛,266003 |
| 基金项目： | 国家自然科学基金资助项目（30471899） |
| 摘 要： | 目的研究富血小板血浆(platelet-rich plasma,PRP)对犬骨髓间充质细胞(marrow stromal cells,MSCs)碱性磷酸酶(alka-line phosphatase,ALP)及矿化结节形成能力的影响。方法将不同浓度(10%、20%、30%)的PRP及重组人骨形成蛋白(rhBMP-2)加入体外培养的第3代MSCs中,对照组为无血清培养液,检测其对细胞ALP活性的影响;将第3代的MSCs在体外进行培养,实验组加入矿化液和PRP(10%或30%),对照组只加矿化液。4周后用茜素红及Von-Kossa染色检测矿化结节的形成。结果10%PRP和rhBMP-2能显著提高MSCs的ALP活性,其中10%PRP对体外矿化结节的形成,亦有一定程度的促进作用。结论PRP有促进MSCs向成骨细胞方向转化的趋势。 |
| 关 键 词： | 富血小板血浆 骨髓间充质细胞 碱性磷酸酶 矿化 犬 |
| 文章编号： | 1003-9872(2007)05-0232-04 |
| 修稿时间： | 2007-01-18 |
Effects of platelet-rich plasma on mineral characteristics of bone marrow stromal cells |
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| PAN Yong-hai,LI Ning-yi,QIU Jian-zhong,JIN Xiao-ming,CHEN Li-qiang,LIU Xiang-ping,SUI Ai-hua. Effects of platelet-rich plasma on mineral characteristics of bone marrow stromal cells[J]. Stomatology, 2007, 27(5): 232-235 | |
| Authors: | PAN Yong-hai LI Ning-yi QIU Jian-zhong JIN Xiao-ming CHEN Li-qiang LIU Xiang-ping SUI Ai-hua |
| Affiliation: | Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Medical College, Qingdao University; Qingdao Central Hospital, Qingdao 266042, China |
| Abstract: | Objective To investigate the effects of platelet-rich plasma(PRP) on the alkaline phosphatase(ALP) activity and mineralized nodule formation of dog's bone marrow stromal cells(MSCs). Methods Various concentrations of PRP(0,10%,20%,30%) and rhBMP-2 were added to the medium of cultured third passage MSCs. After 3,6,9,12 days of co-incubation, the ALP activity was measured. After the third passage MSCs was cultured for 4 weeks, mineralized solution and PRP(10%,30%) were added into the test group medium. Mineralized solution was added into the medium of the control group. At day 28,the formation of mineralized nodules was detected with Dahl McGec-Russll's alizarin red stain and von Kossa's silver nitrate for calcium. Results PRP at the concentration of 10% could increase ALP activity; the 10%PRP had an optimal effect(P<0.05).PRP could also induce nodule formation to some degree. Conclusion PRP has the tendency of inducing MSCs to differentiate into osteoblastic cells. |
| Keywords: | platelet-rich plasma bone marrow stromal cells alkaline phosphatase mineralization dogs |
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