| 不同产地麦冬的指纹图谱比较研究 |
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| 引用本文： | 林以宁, 志田保夫, 袁博, 程志红, 余伯阳, 朱丹妮. 不同产地麦冬的指纹图谱比较研究[J]. 中国药科大学学报, 2005, 36(6): 538-542. |
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| 作者姓名： | 林以宁 志田保夫 袁博 程志红 余伯阳 朱丹妮 |
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| 作者单位： | 1.中国药科大学现代中药教育部重点实验室;2.中国药科大学中药复方研究室;3.东京药科大学 |
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| 摘 要： | 目的:建立麦冬两大主产区川麦冬、杭麦冬的正丁醇和乙醚部位的指纹图谱,比较川麦冬、杭麦冬皂苷和黄酮化学成分的异同。方法:分别采用HPLC-ELSD、ESI/TOF-MS方法建立正丁醇部位指纹图谱;以HPLC-UV法建立乙醚部位指纹图谱。结果:在对皂苷部位的检测分析中,HPLC-ELSD、ESI/TOF-MS两种方法分别从不同角度反映了样品的内在信息,川麦冬、杭麦冬的HPLC-ELSD的指纹图谱虽存在共有峰,但谱图整体存在一定的差异;ESI/TOF-MS显示两者在MS1m/z上重叠性较高,在皂苷化学成分“质”上具有较高相似性。HPLC-UV指纹分析表明两者在成分相对集中的谱图区域具有较高相似性。结论:川麦冬、杭麦冬的皂苷和黄酮化学成分具有较高的相似性,HPLC-ELSD、HPLC-UV指纹图谱可用以区别这两个产地的麦冬。
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| 关 键 词： | 麦冬 指纹图谱 皂苷 黄酮 HPLC-ELSD ESI/TOF-MS HPLC-UV 比较 |
| 文章编号： | 1000-5048(2005)06-0538-05 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-06-30 |
Comparative Studies on Fingerprints of Root of Ophiopogon japonicus Cultivated in Different Areas |
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| LIN Yi-Ning,SHIDA Yasuoao,YUAN Bo,CHENG Zhi-Hong,YU Bo-Yang,ZHU Dan-Ni. Comparative Studies on Fingerprints of Root of Ophiopogon japonicus Cultivated in Different Areas[J]. Journal of China Pharmaceutical University, 2005, 36(6): 538-542 |
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| Authors: | LIN Yi-Ning SHIDA Yasuoao YUAN Bo CHENG Zhi-Hong YU Bo-Yang ZHU Dan-Ni |
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| Abstract: | AIM:To establish the fingerprints for characterization of the chemical components of maidong(root of Ophiopogon japonicus)in sichuan(Chuanmaidong) and hangzhou(Hangmaidong).METHODS:HPLC-ELSD and ESI/TOF-MS analytical methods were applied to detect n-butanol extracts respectively,and HPLC-UV method was employed to study ether extracts.RESULTS:HPLC-ELSD and ESI/TOF-MS methods provided a wide range of the chemical information of n-butanol extracts.HPLC-ELSD fingerprint patterns,as a whole,were different between Chuanmaidong and Hangmaidong although they shared several mutual peaks.However,ESI/TOF-MS analysis showed close similarity between Chuanmaidong and Hangmaidong in the MS~(1) scan.On the other hand,HPLC-UV fingerprint revealed similarity in the second half of the fingerprint pattern.CONCLUSION:Most saponins and flavonoids contained in the two cultivating areas are similar.Meanwhile,the fingerprint developed by HPLC-ELSD and HPLC-UV methods could differentiate the cultivating areas of the two maidong. |
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| Keywords: | HPLC-ELSD ESI/TOF-MS HPLC-UV |
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