| 贝前列素钠预处理对兔心肌缺血/再灌注损伤的早期拮抗作用 |
| |
| 引用本文： | 杨雪,王晓云,母志睿,路英杰,张旭昌. 贝前列素钠预处理对兔心肌缺血/再灌注损伤的早期拮抗作用[J]. 心脏杂志, 2011, 23(4): 434-437. DOI: 61-1268/R.20110503.1152.005 |
| |
| 作者姓名： | 杨雪 王晓云 母志睿 路英杰 张旭昌 |
| |
| 作者单位： | 1.哈尔滨医科大学附属第四医院心血管内科，黑龙江 哈尔滨 150001；2.太仓市第一人民医院心内科，江苏 太仓 215400 |
| |
| 摘 要： | 目的:探讨贝前列素钠（beraprost sodium，BPS）预处理对兔心肌缺血/再灌注(I/R)损伤的早期拮抗作用。方法: 将36只日本大耳白兔随机分为3组，即A组（假手术组）、B组（I/R组:缺血40 min后，再灌注90 min）和C组（BPS预处理组:以15 μg/kg，2次/d，［1］连续喂养4周，最后1次喂养后3 h内进行I/R）。以上3组均开胸暴露心脏，A组在左冠状动脉左室支处穿线不结扎，观察130 min结束实验；B组及C组开胸后结扎左冠状动脉左室支40 min，心电图若出现Ⅱ导联ST段升高及结扎周围心肌的颜色变紫或变深显示已形成心肌梗死(MI)模型。然后进行以下检测:①再灌注90 min后，随机选取6只日本大耳白兔，立即取结扎点下损伤的心肌组织，用100 g/L福尔马林固定后，部分进行HE染色在光镜下观察心肌形态学改变；部分用免疫组化染色法检测心肌细胞中Bax、Bcl-2的表达。②再灌注4 h后，取右心房（或股静脉）血应用电化学发光免疫法检测血浆中心肌中肌酸激酶(CK-MB)的变化。结果: ①HE染色显示，A组的心肌结构正常，B组的心肌损伤较严重，C组的心肌损伤较轻。②免疫组化染色法检测显示，A组Bcl-2的表达呈阳性，Bax的表达呈阴性；B组Bax的表达呈强阳性，Bcl-2的表达呈弱阳性；C组Bax的表达呈弱阳性，Bcl-2的表达介于A组与B组之间。③用电化学发光免疫法检测心肌酶学结果，CK-MB升高者3个组依次为B组>C组>A组，B组CK-MB的水平均明显高于C组与A组（P<0.01）。结论: BPS预处理对兔心肌I/R损伤早期具有拮抗作用。
|
| 关 键 词： | 贝前列素钠 药物预处理 缺血/再灌注损伤 兔 |
| 收稿时间： | 2010-09-21 |
Early antagonism of beraprost sodium pretreatment on ischemic/reperfusion injury in rabbit myocardium |
| |
| YANG Xue,WANG Xiao-yun,MU Zhi-rui,LU Ying-jie,ZHANG Xu-chang. Early antagonism of beraprost sodium pretreatment on ischemic/reperfusion injury in rabbit myocardium[J]. Chinese Heart Journal, 2011, 23(4): 434-437. DOI: 61-1268/R.20110503.1152.005 |
| |
| Authors: | YANG Xue WANG Xiao-yun MU Zhi-rui LU Ying-jie ZHANG Xu-chang |
| |
| Affiliation: | YANG Xue,WANG Xiao-yun,MU Zhi-rui,LU Ying-jie,ZHANG Xu-chang(Department of Cardiology,Fourth Hospital,Harbin Medical University,Harbin 150001,Heilongjiang,China) |
| |
| Abstract: | AIM:To investigate the early antagonism of beraprost sodium (BPS) preconditioning on ischemic/reperfusion (I/R) injury in rabbit myocardium. METHODS: Thirty-six Japanese white rabbits were randomized into three groups: (A) sham operation group (thoracotomy and threading around left coronary artery without ligation), observation for 130 min, (B) I/R group (thoracotomy with left coronary artery ligation for 40 min and reperfusion for 90 min) and (C) BPS pretreatment group (BPS, 15 μg/kg, twice a day for 4 weeks, I/R within 3 h after the last administration). Group B and group C underwent the same time length of ischemia/reperfusion. After 90 min reperfusion, six rabbits were randomly selected for HE coloration and immunohistochemical detection of Bax and Bcl-2. After 4 h reperfusion, CK-MB in plasma was detected. RESULTS: HE stain showed that the myocardial structure was normal in group A, severely injured in group B and mildly in group C. Immunohistochemical stain showed that the expression of Bax was negative, whereas Bcl-2 was positive in group A. Bax was positive, whereas Bcl-2 was weakly positive in group B. Bax was weakly positive, whereas Bcl-2 was between that in group A and group B. There was an increase in CK. Group B>group C>group A. CONCLUSION: BPS may provide some early antagonism on ischemic/reperfusion injury in rabbit myocardium in vivo. |
| |
| Keywords: | beraprost sodium drug preconditioning ischemic reperfusion injury rabbit |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《心脏杂志》浏览原始摘要信息 |
|
点击此处可从《心脏杂志》下载免费的PDF全文 |
|
