| 胡椒碱减轻兔原代左心房肌细胞氧化应激损伤的作用 |
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| 引用本文： | 田苗,刘屏,王字玲,官媛,刘岩,王玉堂,单兆亮. 胡椒碱减轻兔原代左心房肌细胞氧化应激损伤的作用[J]. 心脏杂志, 2011, 23(4): 426-428,441. DOI: 61-1268/R.20110503.1149.003 |
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| 作者姓名： | 田苗 刘屏 王字玲 官媛 刘岩 王玉堂 单兆亮 |
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| 作者单位： | 解放军总医院:1.心内科，2.药理研究室，北京 100853；3.军事医学科学院野战输血研究所，北京 100850 |
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| 基金项目： | 国家自然科学基金项目资助(30772886) |
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| 摘 要： | 目的:利用低浓度过氧化氢（H2O2）建立兔原代左心房肌细胞氧化损伤模型，探讨胡椒碱减轻氧化应激损伤的保护作用。方法: 将18只新西兰白兔随机分为3组，每组6只:即正常对照(NC)组、H2O2组和胡椒碱组，均分别进行左心房肌细胞的原代培养。NC组对培养的心房细胞直接进行检测，H2O2组直接在培养的原代心房肌细胞中加入终浓度为100 μmol/L的H2O2培养2 h，胡椒碱组以7×10-6 mol/L浓度的胡椒碱处理细胞1 h之后，加入终浓度为100 μmol/L的H2O2共同培养2 h。检测氧化和抗氧化指标的变化。MTT法检测三组原代细胞活力，黄嘌呤氧化酶法检测超氧化物歧化酶（SOD）活力、比色法检测丙二醛（MDA）含量及还原型谷胱甘肽（GSH）含量，Fura-2 AM检测细胞内钙离子浓度，RT-PCR对线粒体mRNA进行定量分析。结果: 与NC组相比较，H2O2组细胞的活力、SOD的活力及GSH的含量明显下降（P<0.05）； MDA的含量、钙离子浓度和线粒体mRNA的表达均明显增加（P<0.05）。胡椒碱组和H2O2组比较，上述指标均有显著改善（P<0.05）。结论: 胡椒碱能够在氧自由基的产生清除等环节，减轻兔原代左心房肌细胞的氧化应激损伤。
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| 关 键 词： | 胡椒碱 心房 原代细胞 氧化应激 过氧化氢 |
| 收稿时间： | 2011-01-29 |
Protective effects of piperine against oxidative stress in rabbit primary left atrial cells |
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| TIAN Miao,LIU Ping,WANG Zi-ling,GUAN Yuan,LIU yan,WANG Yu-tang,SHAN Zhao-liang. Protective effects of piperine against oxidative stress in rabbit primary left atrial cells[J]. Chinese Heart Journal, 2011, 23(4): 426-428,441. DOI: 61-1268/R.20110503.1149.003 |
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| Authors: | TIAN Miao LIU Ping WANG Zi-ling GUAN Yuan LIU yan WANG Yu-tang SHAN Zhao-liang |
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| Affiliation: | TIAN Miao1,LIU Ping2,WANG Zi-ling3,GUAN Yuan1,LIU yan1,WANG Yu-tang1,SHAN Zhao-liang1(1.Department of Cardiology,2.Department of Clinical Pharmacology and Pharmacy,PLA General Hospital,Beijing 100853,China,3.Institute of Blood Transfusion Medicine,Academy of Military Medical Sciences,Beijing 100850,China) |
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| Abstract: | AIM:To evaluate the injury caused by low concentration hydrogen peroxide (H2O2) in primary left atrial cells and the protective effect of piperine in rabbits. METHODS: Eighteen rabbits were randomly divided into three groups: normal control (NC) group, H2O2 group and piperine group. Left atrial cells were primarily cultured. In H2O2 group, 100 μmol/L H2O2 was added into culture medium for 2 hours. In piperine group, 7×10-6 mol/L piperine was added for 1 hour and then 100 μmol/L H2O2 was added for 2 hours. The parameters of oxidation and antioxidation were measured. RESULTS: In H2O2 group, cell viability, activity of superoxide dismutase (SOD) and content of glutathione hormone (GSH) decreased (P<0.05). But content of malondialdehyd (MDA), intracellular free calcium concentration and expression of mitochondrial mRNA increased obviously (P<0.05). Compared with those in the H2O2 group, these parameters improved significantly (P<0.05) in the piperine group. CONCLUSIONS: Piperine protects the oxidative stress induced by H2O2 in rabbit primary left atrial cells. |
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| Keywords: | piperine atrium primary cell oxidative stress hydrogen peroxide |
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