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| 小分子化合物S1抑制K562细胞增殖及其机理研究 | |
| 引用本文： | 陈文娟1,韩 乐2,锻 炼3,张寅斌4,赵 征1. 小分子化合物S1抑制K562细胞增殖及其机理研究[J]. 现代肿瘤医学, 2018, 0(15): 2349-2351. DOI: 10.3969/j.issn.1672-4992.2018.15.008 |
| 作者姓名： | 陈文娟1 韩 乐2 锻 炼3 张寅斌4 赵 征1 |
| 作者单位： | 1.陕西省肿瘤医院内三科；2.胸外科，陕西 西安 710061；3.空军军医大学唐都医院肿瘤科，陕西 西安 710038；4.西安交通大学第二附属医院肿瘤内科，陕西 西安 710004 |
| 摘 要： | 目的:探讨小分子化合物S1对K562细胞的抑制作用及机理，为S1治疗慢性粒细胞白血病（chronic myeloid leukemia，CML）提供理论与实验基础。方法:MTT法检测不同浓度S1对K562细胞增殖的抑制作用；应用Chou-Talalay计算联合指数，观察单独应用S1与S1联合阿糖胞苷对K562细胞增殖抑制的协同作用；Western-blotting方法检测不同浓度S1作用后K562细胞系中Bcl-2蛋白表达水平的变化。结果:与对照组相比，S1可以抑制K562细胞的增殖（P<0.05）；S1与阿糖胞苷联合应用对K562细胞的生长抑制具有协同作用，联合指数CI<1；S1可下调K562细胞系中抗凋亡蛋白Bcl-2的表达。结论:小分子化合物可通过下调抗凋亡蛋白Bcl-2的表达抑制K652细胞的增殖，并与阿糖胞苷具有协同作用。 |
| 关 键 词： | 小分子化合物S1 慢性粒细胞白血病 Bcl-2蛋白 |
The study of effect and mechanism of small molecular compound S1 inhibiting proliferation of K562 cell |
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| Chen Wenjuan1,Han Le2,Duan Lian3,Zhang Yinbin4,Zhao Zheng1. The study of effect and mechanism of small molecular compound S1 inhibiting proliferation of K562 cell[J]. Journal of Modern Oncology, 2018, 0(15): 2349-2351. DOI: 10.3969/j.issn.1672-4992.2018.15.008 | |
| Authors: | Chen Wenjuan1 Han Le2 Duan Lian3 Zhang Yinbin4 Zhao Zheng1 |
| Affiliation: | 1.The Third Department of Internal Medicine；2.Department of Thoracic Surgery,Shaanxi Provincial Cancer Hospital，Shaanxi Xi'an 710061，China；3.Department of Oncology，Tangdu Hospital，Air Force Medical University，Shaanxi Xi'an 710038，China；4.Department of Medical Oncology，the Second Affiliated Hospital of Xi'an Jiaotong University，Shaanxi Xi'an 710004，China. |
| Abstract: | Objective:To investigate its therapeutic potential as a novel small-molecule inhibitor of Bcl-2 for the treatment of chronic myeloid leukemia (CML).Methods:K562 cells proliferation was measured by MTT assay.We used the Chou-Talalay method to determine the compound combination synergism.The expression of Bcl-2 protein was examined with Western-blotting.Results:Compared with control group，S1 can decrease the survival rate of K562 cells （P<0.05）.S1 showed a synergistic effect when combined with cytosine arabinoside hydrochloride (Ara-c) in inhibiting K562 cell proliferation，CI<1.S1 down-regulated the Bcl-2 protein in K562 cell line.Conclusion:S1 can inhibit proliferation of K562 cell through down-regulating the expression of Bcl-2 protein.Meanwhile，it can demonstrate a synergistic combination effect with cytosine arabinoside. |
| Keywords: | S1 CML Bcl-2 protein |
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