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| CRISPR-Cas9基因编辑技术在眼科疾病中的应用 | |
| 引用本文： | 管丽红,林俊堂. CRISPR-Cas9基因编辑技术在眼科疾病中的应用[J]. 眼科新进展, 2018, 0(9): 801-803. DOI: 10.13389/j.cnki.rao.2018.0190 |
| 作者姓名： | 管丽红 林俊堂 |
| 作者单位： | 453003 河南省新乡市，新乡医学院生命科学技术学院干细胞中心，河南省医用组织再生重点实验室（管丽红，林俊堂）；453003 河南省新乡市，新乡医学院生物医学工程学院（林俊堂） |
| 摘 要： | 规律成簇间隔短回文重复（clustered regularly interspaced short palindromic repeat,CRISPR）及相关核酸内切酶9（CRISPR associated protein,Cas9）技术是一种由RNA指导核酸内切酶的基因编辑技术。该技术以其操作简便、基因敲除效率高、靶向精准、周期短等特点迅速被用于多个物种的基因组编辑及疾病基因治疗中。本文旨在对CRISPR-Cas9技术在构建眼科疾病模型和治疗眼科疾病中的应用进展进行综述。 |
| 关 键 词： | CRISPR-Cas9 基因组编辑 基因治疗 眼科疾病 |
Application of CRISPR-Cas9 technology in ophthalmological diseases |
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| GUAN Li-Hong,LIN Jun-Tang. Application of CRISPR-Cas9 technology in ophthalmological diseases[J]. Recent Advances in Ophthalmology, 2018, 0(9): 801-803. DOI: 10.13389/j.cnki.rao.2018.0190 | |
| Authors: | GUAN Li-Hong LIN Jun-Tang |
| Affiliation: | Stem Cell Center,College of Life Science and Technology,Xinxiang Medical University，Henan Key Laboratory of Medical Tissue Regeneration（GUAN Li-Hong,LIN Jun-Tang），Xinxiang 453003,Henan Province,China；College of Biomedical Engineering,Xinxiang Medical University（LIN Jun-Tang）,Xinxiang 453003,Henan Province,China |
| Abstract: | The clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR associated protein 9 (Cas9) is a gene editing technology directed by RNA endonuclease.This technology is rapidly used in genome editing and gene therapy for many species because of its simple operation,high gene knockout efficiency,precise target and short cycle.The purpose of this paper is to review the application and progress of CRISPR-Cas9 technology in the construction of ophthalmological disease models and the treatment of ophthalmological diseases. |
| Keywords: | CRISPR-Cas9 genome editing gene therapy ophthalmological diseases |
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