| 沉默信息调节因子-1及上皮性钙黏附素在胃癌中的表达及其相关性 |
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| 引用本文： | 李明辉1,赵静雅2,董永杰1,李玉梅1,冯运章1,霍 双3. 沉默信息调节因子-1及上皮性钙黏附素在胃癌中的表达及其相关性[J]. 现代肿瘤医学, 2018, 0(12): 1888-1892. DOI: 10.3969/j.issn.1672-4992.2018.12.021 |
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| 作者姓名： | 李明辉1 赵静雅2 董永杰1 李玉梅1 冯运章1 霍 双3 |
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| 作者单位： | 1.邯郸市中心医院普外二科;2.病理科;3.科研科，河北 邯郸 056001 |
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| 基金项目： | 河北省科技厅重点研发计划项目（编号:162777224） |
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| 摘 要： | 目的:探讨沉默信息调节因子-1(silence signal regulating factor-1，SIRT-1)和上皮性钙黏附素(epithelial cadherin，E-cadherin)在胃癌组织中的表达及其相关性。方法:采用HE 染色观察正常胃黏膜组织及癌组织的病理结构；采用免疫组织化学方法检测SIRT-1和E-cadherin在正常胃黏膜组织及癌组织中的表达水平;Spearman相关检验检测SIRT-1 与E-cadherin 表达的相关性。结果:HE 染色结果:胃癌组织中癌细胞分化差，多形性，大小不一，可呈假复层，核大，胞浆少，容易找到核分裂。免疫组织化学检测结果:SIRT-1在正常胃黏膜组织和胃癌组织的阳性表达率分别为40.5%和64.3%;E-cadherin 的阳性表达率分别为73.8%和40.5%。两组SIRT-1和E-cadherin的阳性表达率差异有统计学意义（P<0.05）。Spearman相关分析显示SIRT-1 与E-cadherin 的表达水平呈负相关（P<0.05）。胃癌组织中SIRT-1蛋白阳性表达与肿瘤大小及病理类型有关（P<0.05）；而与患者的年龄、性别、TNM分期及淋巴结转移无关（P>0.05）。结论:胃癌组织中SIRT-1高表达可能通过抑制E-cadherin促使肿瘤组织细胞发生侵袭、转移。
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| 关 键 词： | 胃癌 沉默信息调节因子-1 上皮性钙黏附素 免疫组织化学 |
The expressions and relationship of SIRT-1 and E-cadherin in gastric cancer |
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| Li Minghui1,Zhao Jingya2,Dong Yongjie1,Li Yumei1,Feng Yunzhang1,Huo Shuang3. The expressions and relationship of SIRT-1 and E-cadherin in gastric cancer[J]. Journal of Modern Oncology, 2018, 0(12): 1888-1892. DOI: 10.3969/j.issn.1672-4992.2018.12.021 |
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| Authors: | Li Minghui1 Zhao Jingya2 Dong Yongjie1 Li Yumei1 Feng Yunzhang1 Huo Shuang3 |
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| Affiliation: | 1.Department of Second General Surgery；2.Department of Pathology;3.Department of Scientific Research,Handan Central Hospital，Hebei Handan 056001,China. |
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| Abstract: | Objective:To research the expressions and relationship of silence signal regulating factor-1(SIRT-1) and epithelial cadherin(E-cadherin) in gastric cancer.Methods:HE staining was used to observe the histopathologic structure in the cancer group and normal gastric mucosa group.Immunohistochemistry SP technique was used to detect the expressions of SIRT-1 and E-cadherin in cancer group and normal gastric mucosa group.Spearman correlation test was used to detect the expression correlation of SIRT-1 and E-cadherin.Results:HE staining gastric cancer tissue:Cell differentiation was poor,pleomorphism and different size.Cells showed fake many layers,cell nucleus was large and karyokinesis,kytoplasm was few.The immunohistochemistry showed the positive expression of SIRT-1 were 40.5% and 64.3% in the normal gastric mucosa group and cancer group.The positive expression rate of E-cadherin were 73.8% and 40.5% respectively in the normal gastric mucosa group and cancer group(P<0.05).Spearman correlation test showed that there was a correlation between SIRT-1 and E-cadherin(P<0.05).SIRT-1 protein expression was related to the tumor size and pathological types(P<0.05).But not related to the age,gender，TNM stage and lymph node metastasis(P>0.05).Conclusion:Gastric cancer with the higher SIRT-1 expression may be the ways to achieve tumor invasion and metastasis through E-cadherin as a facilitator. |
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| Keywords: | gastric cancer SIRT-1 E-cadherin immunohistochemistry |
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