Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species |
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| Authors: | Russo Cristina Tortoli Enrico Menichella Donato |
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| Affiliation: | Centro Regionale di Riferimento per la Diagnostica dei Micobatteri, Piastra dei Servizi, Ospedale di Careggi, viale Morgagni 85, 50134 Firenze, Italy. |
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| Abstract: | The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more frequently detected mycobacterial species and GenoType AS for less common species. The sensitivity and specificity were 97.9% and 92.4% for the CM kit and 99.3% and 99.4% for the AS kit. Our results show that the system may represent a useful tool to substantially enlarge the number of mycobacteria that can be reliably identified in clinical laboratories. |
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