| 高效液相色谱法测定人血浆中的百草枯 |
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| 引用本文： | 张静1,陈亚玲2,宋科曦3,李树华1,巫波1,李政1,别明江1,朱月武1. 高效液相色谱法测定人血浆中的百草枯[J]. 现代预防医学, 2016, 0(17): 3203-3206 |
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| 作者姓名： | 张静1 陈亚玲2 宋科曦3 李树华1 巫波1 李政1 别明江1 朱月武1 |
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| 作者单位： | 1.四川大学华西第四医院检验科,四川 成都 610041;2.四川大学华西公共卫生学院(华西第四医院),四川 成都 610041;3四川省绵竹市疾病预防控制中心,四川 绵竹,618200 |
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| 摘 要： | 目的 建立人血浆中百草枯的高效液相色谱快速测定方法。方法 用正交试验优化色谱条件,流动相为甲醇-缓冲液(10:90,v/v),缓冲液为20 mmol/L的十二烷基庚烷磺酸钠(含20 mmol/L磷酸,用三乙胺调节pH为3.5),检测波长:256 nm,流速:0.80 ml/min,柱温为室温。样品依次用乙腈、二氯甲烷处理沉淀血浆中蛋白质并除去脂溶性杂质,取20 μl上清液进样测定。结果 血浆百草枯在0.1 μg/ml到10 μg/ml范围内线性关系良好,回归方程为y= 6442.6x + 44.47(r = 0.9992),平均回收率为93.8％-101.1％,日内RSD为3.08％,日间RSD为3.92％。结论 该方法简便快速、准确灵敏,适用于人血浆中百草枯快速检测,为百草枯中毒患者的临床诊断提供参考依据。
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| 关 键 词： | 百草枯 高效液相色谱法 血浆 |
Determination of paraquat in human plasma by high performance liquid chromatography |
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| ZHANG Jing,CHEN Ya-ling,SONG Ke-xi,LI Shu-hua,WU Bo,LI Zheng,BIE Ming-jiang,ZHU Yue-wu. Determination of paraquat in human plasma by high performance liquid chromatography[J]. Modern Preventive Medicine, 2016, 0(17): 3203-3206 |
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| Authors: | ZHANG Jing CHEN Ya-ling SONG Ke-xi LI Shu-hua WU Bo LI Zheng BIE Ming-jiang ZHU Yue-wu |
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| Affiliation: | *No.4 West China Teaching Hospital ,Sichuan University,No 18,Section 3,South Renmin Road,Chengdu,Sichuan 610041,China |
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| Abstract: | Objective To establish a quick analytical method for determination of paraquat in human plasma by HPLC. Methods The chromatographic conditions were optimized using orthogonal test. The mobile phase consisted of methanol-water phase containing 20 mmol/L sodium dodecyl heptane and 20 mmol/L phosphoric acid (10:90v/v,pH=3.5 adjusted with triethylamine). The detection wavelength was set at 256 nm. The flow rate was 0.8 ml/min. The column temperature was at the room temperature. The protein and fat-soluble interferents in samples were precipitated and removed with acetonitrile and methylene chloride in sequence. Results The calibration curve was linear in the range of 0.1-10 μg/ml,and the regression equation was y=6442.6x+44.47(r=0.9992). The average recoveries were in the range of 93.8％-101.1％,the intra-day RSD was 3.08％,and the inter-day RSD was 3.92％. Conclusion The method is simple,rapid,accurate and sensitive,and it can be applied for the determination of paraquat in human plasma,providing a reference for the diagnosis of paraquat poisoning. |
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| Keywords: | Paraquat High performance liquid chromatography Plasma |
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