| 饮料、发酵酒及调味品中L-谷氨酰胺的高效液相色谱测定法 |
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| 引用本文： | 向仲朝,杨文婉,李佳佳. 饮料、发酵酒及调味品中L-谷氨酰胺的高效液相色谱测定法[J]. 现代预防医学, 2016, 0(15): 2736-2738 |
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| 作者姓名： | 向仲朝 杨文婉 李佳佳 |
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| 作者单位： | 绵阳市疾病预防控制中心,四川 绵阳 621000 |
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| 摘 要： | 目的 建立发酵酒、饮料及调味品中L-谷氨酰胺测定的高效液相色谱法。方法 样品用水或流动相稀释或超声提取,采用Zorbax SB-18为分离柱,以戊烷磺酸钠溶液为流动相,检测波长为210nm。结果 本法L-谷氨酰胺在0 mg/mL～1.00 mg/mL范围内,线性关系良好（r=0.999 97）;方法检出限0.40mg/kg。样品中L-谷氨酰胺测定值为44.2mg/kg～486.0mg/kg,相对标准偏差（RSD）为2.1%～5.6%。饮料、啤酒、葡萄酒等样品提取液中加标量为0.20 mg/mL～0.80mg/mL时,回收率为87.8%～115.0%,平均回收率为92.7%。结论 本法样品处理简单,操作方便,测定食品中L-谷氨酰胺的含量快速、精密、准确。
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| 关 键 词： | L-谷氨酰胺 高效液相色谱法 食品 |
Determination of L-glutamine in the fermented wine,drink and spice by high-performance liquid chromatography |
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| XIANG Zhong-zhao,YANG Wen-wan,LI Jia-jia. Determination of L-glutamine in the fermented wine,drink and spice by high-performance liquid chromatography[J]. Modern Preventive Medicine, 2016, 0(15): 2736-2738 |
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| Authors: | XIANG Zhong-zhao YANG Wen-wan LI Jia-jia |
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| Affiliation: | Mianyang Centre of Disease Control and Prevention,Mianyang,Sichuan 621000,China |
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| Abstract: | Objective To establish a high-performance liquid chromatography (HPLC) method for determination of L-glutamine in fermented wine,drink,and spice. Methods L-glutamine in solid samples were extracted ultrasonically with water or mobile phase and then filtrated.But for a liquid sample,it could be diluted with water or mobile phase and then filtrated.The sample solution was finally determined with a Zorbax SB-18 column using gradient elution with sodium pentanesulfonate solution as the mobile phase.The detection wavelength was set at 210nm. Results The linear range of the method was 0 mg/Ml~1.00 mg/ml.The detection limit was 0.40 mg/kg.The concentrations of L-glutamine detected in samples ranged from 44.2 mg/kg~486mg/kg.The relative standard deviations ranged from 2.1%~5.6%.When spiked from 0.20 mg/Ml~0.80mg/mL,the recoveries were in the range of 87.8%~115%,with the average recovery of 92.7%. Conclusion The method is simple,accurate and rapid for the determination of L-glutamine in food. |
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| Keywords: | L-glutamine High-performance liquid chromatography Food |
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