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| 碱性成纤维细胞生长因子在大鼠坐骨神经分支选择性损伤后的动态变化 | |
| 引用本文： | 游霁月,高进,陈萍,孙琳,杨晓秋. 碱性成纤维细胞生长因子在大鼠坐骨神经分支选择性损伤后的动态变化[J]. 南方医科大学学报, 2013, 33(4): 563-567 |
| 作者姓名： | 游霁月 高进 陈萍 孙琳 杨晓秋 |
| 作者单位： | 重庆医科大学附属第一医院麻醉科,重庆,400016 |
| 基金项目： | 重庆市卫生局医学科研计划项目 |
| 摘 要： | 目的观察坐骨神经分支选择性损伤（SNI）大鼠机械刺激缩足反射阈值（PWMT）与脊髓碱性成纤维细胞生长因子 （FGF-2）、肿瘤坏死因子（TNF）-α和白介素（IL）-6表达变化的时程关系，探讨神经病理性疼痛中脊髓FGF-2的动态变化规律及 致痛机制。方法雄性SD大鼠100只，采用随机数字表法，将其随机分2组（n=50）：假手术组（Sh组）和手术组（SNI组）。于术前 1 d，术后1、4、7、14、28 d 观察疼痛行为学，检测机械刺激缩足反射阈值（PWMT），并于术后各时间点应用免疫组织化学、 Western blot以及ELISA方法，检测脊髓FGF-2、TNF-α和IL-6含量。结果SNI后1 d PWMT下降，7 d达最低，28 d仍处于较低 水平，与Sh组术后各时间点和术前比较差异显著（P<0.05）。SNI组脊髓FGF-2含量于术后4 d开始增加，14 d达高峰，28 d仍维 持较高水平，与Sh组术后各时间点比较差异显著（P<0.05）。SNI组术后各时间点TNF-α和IL-6的表达高于Sh组（P<0.05）。结 论大鼠SNI模型成功模拟神经病理性疼痛的发生，并且诱发损伤侧脊髓FGF-2以及炎性因子表达增加。 |
| 关 键 词： | 碱性成纤维细胞生长因子 坐骨神经分支选择性损伤 神经病理性疼痛 炎性因子 脊髓 |
Changes of basic fibroblast growth factor expression in the spinal cord of rats with spared nerve injury of the sciatic nerve |
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| YOU Jiyue , GAO Jin , CHEN Ping , SUN Lin , YANG Xiaoqiu. Changes of basic fibroblast growth factor expression in the spinal cord of rats with spared nerve injury of the sciatic nerve[J]. Journal of Southern Medical University, 2013, 33(4): 563-567 | |
| Authors: | YOU Jiyue GAO Jin CHEN Ping SUN Lin YANG Xiaoqiu |
| Affiliation: | Department of Anesthesiology,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China |
| Abstract: | Objective To observe the temporal changes of basic fibroblast growth factor (FGF-2) expression in the spinal cord of a rat model of spared nerve injury (SNI) of the sciatic nerve. Methods A total of 100 male SD rats were randomly divided into sham-operated group and SNI group. The paw withdrawal threshold to mechanical stimulation was recorded at 1 day before and at 1, 4, 7, 14 and 28 days after the operation. The expressions of FGF-2, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the L4-6 spinal cord segments of the rats were measured at the specified time points. Results The paw withdrawal threshold began to decline at 1 day after SNI, reached the lowest level at 7 days, and maintained a low level till 28 days (P< 0.05). The expression of FGF-2 began to increase significantly on postoperative day 4, reached the peak level on day 14 and maintained the high level till day 28 (P<0.05). The rats with SNI showed significantly higher expressions of TNF-α and IL-6A in the spinal cord than those in the sham-operated group at each time point of measurement (P<0.05). Conclusion SNI of the sciatic nerve can induce neuropathic tactile allodynia and causes up-regulation of FGF-2 and inflammatory cytokines in the spinal cord. |
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