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| 钠离子通道阻断剂抑制骨髓间充质干细胞向心肌样细胞的分化 | |
| 引用本文： | 郝春媛,马爱群,王亭忠,刘 瑞. 钠离子通道阻断剂抑制骨髓间充质干细胞向心肌样细胞的分化[J]. 中国组织工程研究, 2013, 17(23): 4181-4188. DOI: 10.3969/j.issn.2095-4344.2013.23.001 |
| 作者姓名： | 郝春媛 马爱群 王亭忠 刘 瑞 |
| 作者单位： | 1西安市第一医院心血管内科,陕西省西安市 7100022西安交通大学医学院第一附属医院心血管内科,陕西省西安市 7100613西安交通大学医学院生理和病理生理学系,陕西省西安市 710061 |
| 基金项目： | 国家自然科学基金项目(81000063)。 |
| 摘 要： | 背景：研究发现人和动物的多种间充质干细胞均存在河豚毒素敏感性钠离子通道,但钠离子通道是否会影响间充质干细胞向心肌细胞分化尚无共识。目的：观察钠离子通道特异性阻断剂河豚毒素对骨髓间充质干细胞向心肌样细胞分化的影响。方法：利用Percoll’s密度梯度离心法结合差速贴壁法分离大鼠骨髓间充质干细胞,用主动脉逆行生物酶灌流法分离大鼠成体心肌细胞。将DAPI标记的大鼠骨髓间充质干细胞与成体心肌细胞混合培养,应用0,10,100 μmol/L的河豚毒素进行干预,1周后观察骨髓间充质干细胞向心肌样细胞的分化情况。结果与结论：免疫荧光细胞化学染色显示,大鼠骨髓间充质干细胞和成体心肌细胞混合培养1周后,骨髓间充质干细胞显著表达心肌特异性蛋白结蛋白和肌球蛋白,而10,100 μmol/L的河豚毒素均可完全抑制结蛋白和肌球蛋白在骨髓间充质干细胞中的表达。提示钠离子通道是骨髓间充质干细胞向心肌样细胞分化的必要条件。 |
| 关 键 词： | 干细胞 骨髓干细胞 骨髓间充质干细胞 心肌损伤 钠离子通道 河豚毒素 心肌样细胞 细胞分化 国家自然科学基金 干细胞图片文章 |
Sodium channel blockers inhibit the differentiation of rat bone marrow mesenchymal stem cells into cardiac-like cells |
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| Hao Chun-yuan,Ma Ai-qun,Wang Ting-zhong,Liu Rui. Sodium channel blockers inhibit the differentiation of rat bone marrow mesenchymal stem cells into cardiac-like cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(23): 4181-4188. DOI: 10.3969/j.issn.2095-4344.2013.23.001 | |
| Authors: | Hao Chun-yuan Ma Ai-qun Wang Ting-zhong Liu Rui |
| Affiliation: | 1 Department of Cardiology, No. 1 Hospital of Xi’an, Xi’an 710002, Shaanxi Province, China 2 Department of Cardiology, First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China 3 Department of Physiology and Pathophysiology, First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China |
| Abstract: | BACKGROUND:The studies have found that the tetrodotoxin-sensitive sodium channels exist in various human and animal mesenchymal stem cells, but whether sodium channels may influence the differentiation of mesenchymal stem cells into myocardial cells has not been confirmed. OBJECTIVE:To observe the effect of sodium channel blocker tetrodotoxin on the differentiation of mesenchymal stem cells into cardiac-like cells. METHODS:Rat bone marrow mesenchymal stem cells were isolated with Percoll’s density gradient centrifugation and differential adhesion method, and then the adult rat myocardial cells were isolated by aorta retrograde collagenase perfusion; rat bone marrow mesenchymal stem cells labeled with 4',6-diamidino-2-phenylindole were co-cultured with adult rat myocardial cells, and induced with 0, 10 and 100 μmol/L tetrodotoxin; and then the differentiation of bone marrow mesenchymal stem cells into cardiac-like cells was observed at 1 week after induction. RESULTS AND CONCLUSION:Immunofluorescence staining showed that the bone marrow mesenchymal stem cells could express cardiac-specific protein desmin and myosin after bone marrow mesenchymal stem cells co-cultured with myocardial cells for 1 week, while 10 and 100 μmol/L tetrodotoxin could inhibit the expressions of desmin and myosin in bone marrow mesenchymal stem cells. The results indicate that sodium channel may play a pivotal role in the differentiation of bone marrow mesenchymal stem cells into cardiac-like cells. |
| Keywords: | stem cells bone marrow-derived stem cells bone marrow mesenchymal stem cells myocardial injury sodium channel tetrodotoxin cardiac-like cells cell differentiation National Natural Science Foundation of China stem cell photographs-containing paper |
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