| 黄芪甲苷联合丹参酮Ⅱa诱导骨髓间充质干细胞向心肌样细胞分化 |
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| 引用本文： | 宋雪云,龙 云,谭 超,郑景辉. 黄芪甲苷联合丹参酮Ⅱa诱导骨髓间充质干细胞向心肌样细胞分化[J]. 中国组织工程研究, 2013, 17(36): 6515-6520. DOI: 10.3969/j.issn.2095-4344.2013.36.021 |
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| 作者姓名： | 宋雪云 龙 云 谭 超 郑景辉 |
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| 作者单位： | 湖南中医药大学第一附属医院,湖南省长沙市 410007;广西中医药大学附属瑞康医院,广西壮族自治区南宁市 530011 |
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| 基金项目： | 国家自然科学基金项目(81102595)*《养心通脉有效部位方对BMSCs作用靶点及信号转导通路的研究》;广西自然科学基金项目(2012GXNSFAA053113) *《心血瘀阻证心肌微环境变化及对骨髓间充质干细胞移植的影响》 |
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| 摘 要： | 背景：黄芪甲苷和丹参酮Ⅱa是传统中医药治疗心肌缺血的有效成分,是否可参与骨髓间充质干细胞向心肌样细胞分化的过程呢？ 目的：观察黄芪甲苷联合丹参酮Ⅱa对体外诱导骨髓间充质干细胞向心肌样细胞分化作用的影响。方法：应用MTT法分别测定黄芪甲苷和丹参酮Ⅱa的最大无毒浓度,确定两者联合诱导骨髓间充质干细胞分化为心肌样细胞的剂量。将分离纯化的骨髓间充质干细胞分5组进行诱导：黄芪甲苷组、丹参酮Ⅱa组、丹参酮Ⅱa+黄芪甲苷组、5-氮胞苷组、空白对照组,ELISA方法观察体外诱导后骨髓间充质干细胞缝隙连接蛋白43和肌钙蛋白表达变化。结果与结论：诱导后丹参酮Ⅱa+黄芪甲苷组、丹参酮Ⅱa、黄芪甲苷、5-氮胞苷组肌钙蛋白、缝隙连接蛋白43表达水平均高于正常对照组(P < 0.01),丹参酮Ⅱa+黄芪甲苷组高于丹参酮Ⅱa、黄芪甲苷组(P < 0.01)。丹参酮Ⅱa+黄芪甲苷组与5-氮胞苷组缝隙连接蛋白43表达水平差异无显著性意义(P > 0.05)。结果提示黄芪甲苷联合丹参酮Ⅱa可以诱导骨髓间充质干细胞向心肌样细胞方向转化,而其联合作用高于单一成分的作用。
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| 关 键 词： | 干细胞 丹参 黄芪 心肌缺血 细胞分化 肌钙蛋白 |
| 收稿时间： | 2013-01-14 |
Combination of tanshinone IIa and astragaloside induces bone marrow mesenchymal stem cells differentiating into myocardium-like cells |
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| Song Xue-yun,Long Yun,Tan Chao,Zheng Jing-hui. Combination of tanshinone IIa and astragaloside induces bone marrow mesenchymal stem cells differentiating into myocardium-like cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(36): 6515-6520. DOI: 10.3969/j.issn.2095-4344.2013.36.021 |
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| Authors: | Song Xue-yun Long Yun Tan Chao Zheng Jing-hui |
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| Affiliation: | The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China; Ruikang Hospital Affiliated to Guangxi University of Chinese Medicining, Nanning 530011, Guangxi Zhuang Autonomous Region, China |
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| Abstract: | BACKGROUND:Astragaloside and tanshinone IIa are the effective components of traditional Chinese medicine treatment of myocardial ischemia, and its role in bone marrow mesenchymal stem cells differentiation into myocardium-like cells remains unclear. OBJECTIVE:To investigate the effect of tanshinone IIa and astragaloside on the differentiation of bone marrow mesenchymal stem cells into myocardium-like cells. METHODS:The maximal non-toxic concentrations of tanshinone IIa and astragaloside were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, to define the dose of the two in the induced differentiation of bone marrow mesenchymal stem cells into myocardium-like cells. The isolated and purified bone marrow mesenchymal stem cells were divided into five groups: astragaloside group, tanshinone IIa group, astragaloside + tanshinone IIa group, 5-azacitidine group, and blank control group. The expression of gap junction connexin 43 and troponin was determined with enzyme-linked immunosorbent assay.RESULTS AND CONCLUSION:The expression of gap junction connexin 43 and troponin in astragaloside group, tanshinone IIa group, astragaloside + tanshinone IIa group, 5-azacitidine group was higher than that in blank control group (P < 0.01). The astragaloside + tanshinone IIa group showed a higher expression of gap junction connexin 43 and troponin than astragaloside group and tanshinone IIa group (P < 0.01). There was no significant difference in the expression of gap junction connexin 43 and troponin between astragaloside + tanshinone IIa group and 5-azacitidine group (P > 0.05). A combined use of astragaloside and tanshinone IIa can induce bone marrow mesenchymal stem cells to differentiate into myocardium-like cells, and their joint role is better than the role of a single ingredient. |
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| Keywords: | stem cells Salvia miltiorrhiza Astragalus membranaceus myocardial ischemia cell |
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