| 长距离跑步大鼠膝关节滑膜基质金属蛋白酶的表达 |
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| 引用本文： | 上官雅娴,卫小春,李 凯,卫建平. 长距离跑步大鼠膝关节滑膜基质金属蛋白酶的表达[J]. 中国组织工程研究, 2013, 17(33): 5909-88. DOI: 10.3969/j.issn.2095-4344.2013.33.002 |
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| 作者姓名： | 上官雅娴 卫小春 李 凯 卫建平 |
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| 作者单位： | 山西医科大学第二医院,1病理科,2骨科,山西省太原市 030001 |
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| 基金项目： | 国家自然科学基金(81000815)：“在体观察过度应力负荷对大鼠关节软骨损伤作用及机制研究”;山西省自然科学基金项目(2010021034-5)：“在体观察过度应力负荷对大鼠关节软骨损伤作用及机制研究”; 山西省卫生厅科技攻关计划项目(2011036),“在体应力负荷对大鼠关节软骨代谢平衡的影响”。 |
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| 摘 要： | 背景：很多研究表明基质金属蛋白酶 1,3,9和13 对关节软骨的退变存在影响,但是针对关节滑膜进行的专项研究相对较少。目的：观察长距离跑步运动对基质金属蛋白酶1,3, 9以及基质金属蛋白酶13在滑膜上的表达的影响。方法：Wistar雄性大鼠15只随机分为3组：对照组、平板组和上坡组。对照组普通笼养;平板组每天在平板0°的水平面跑步机上以1 km/h,运动1 h,持续45 d;上坡组先在平板0°跑步机上以1 km/h,运动1 h,持续15 d,然后在上坡+20°的跑步机上每天以1 km/h,运动1 h,持续30 d。造成不同程度的膝关节滑膜损伤模型,造模成功后取双后肢膝关节,进行石蜡包埋,矢状面整体切片,而后进行苏木精-伊红和免疫组织化学染色,观察并分析实验结果。结果与结论：长距离跑步运动后,平板组和上坡组的滑膜组织中基质金属蛋白酶1的表达都比对照组增高 (P < 0.05),但平板组和上坡组之间差异无显著性意义(P > 0.05);而3组基质金属蛋白酶3的表达并无明显变化(P > 0.05);基质金属蛋白酶9和基质金属蛋白酶13在滑膜组织中的表达呈梯度递增状态(P < 0.05),对照组表达最低,平板组有所升高,上坡组的表达最高。说明长距离跑步运动可通过改变基质金属蛋白酶的表达而影响大鼠膝关节滑膜组织的正常生理结构。
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| 关 键 词： | 组织构建 软骨组织构建 滑膜炎 膝关节 滑膜损伤 基质金属蛋白酶 长距离 跑步运动 免疫组织化学 国家自然科学基金 |
| 收稿时间： | 2013-03-05 |
Expression of matrix metalloproteinases in the synovium of rat knee joint during long-distance running |
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| Shangguan Ya-xian,Wei Xiao-chun,Li Kai,Wei Jian-ping. Expression of matrix metalloproteinases in the synovium of rat knee joint during long-distance running[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(33): 5909-88. DOI: 10.3969/j.issn.2095-4344.2013.33.002 |
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| Authors: | Shangguan Ya-xian Wei Xiao-chun Li Kai Wei Jian-ping |
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| Affiliation: | 1Department of Pathology, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; 2Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China |
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| Abstract: | BACKGROUND: Many studies have shown that matrix metalloproteinases 1, 3, 9 and 13 play an important role in articular cartilage degeneration and destruction, but there is less special research on the articular synovium.OBJECTIVE: To observe the effect of long-distance running on the expressions of matrix metalloproteinases 1, 3, 9 and 13 in the synovium. METHODS:Fifteen male Wistar rats were divided into three groups: control group, tablet group and uphill group. Rats in the control group received ordinary captivity; rats in the tablet group ran on the horizontal treadmill (0°) at the speed of 1 km/h for 1 hour daily, and lasted for 45 days; rats in the uphill group daily ran on the horizontal treadmill (0°) at the speed of 1 km/h for 1 hour, and lasted for 15 days, and then the rats ran on the uphill treadmill (+20°) at the speed of 1 km/h for 1 hour daily and lasted for 30 days. The knee joint synovium injury models with varying degrees were established. The dual hind knee joints were obtained after modeling for paraffin-embedded. Then the overall sagittal slices were obtained for hematoxylin-eosin staining and immunohistochemical staining, and the experimental results were observed and analyzed. RESULTS AND CONCLUSION: After long-distance running, the expression of matrix metalloproteinases 1 in synovium of the tablet group and uphill group was increased when compared with that of the control group (P < 0.05), but there was no significant difference between tablet group and uphill group (P > 0.05). There was no significant difference in matrix metalloproteinases 3 expression (P > 0.05). The expressions of matrix metalloproteinase 9 and matrix metalloproteinase 13 in synovium were in gradient increasing state (P < 0.05), which were lowest in the control group, increased in the tablet group and highest in the uphill group. The results indicate that long-distance running exercise can influence the normal physiological structure of rat knee joint synovium by changing the expression of matrix metalloproteinases. |
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| Keywords: | tissue construction cartilage tissue construction synovitis knee joint synovial injury matrix metalloproteinase long-distance running exercise immunohistochemistry National Natural Science Foundation of China |
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