Comparative analysis of colorimetric staining in skin using open‐source software |
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| Authors: | Paul C. Billings Jenine K. Sanzari Ann R. Kennedy Keith A. Cengel John T. Seykora |
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| Affiliation: | 1. Department of Radiation Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA;2. Department of Dermatology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA |
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| Abstract: | Colorimetric staining techniques such as immunohistochemistry (IHC), immunofluorescence (IF) and histochemistry (HC) provide useful information regarding the localization and relative amount of a molecule/substance in skin. We have developed a novel, straightforward method to assess colorimetric staining by combining features from two open‐source software programs. As a proof of principle, we demonstrate the utility of this approach by analysing changes in skin melanin deposition during the radiation‐induced tanning response of Yucatan mini‐pigs. This method includes a visualization step to validate the accuracy of colour selection before quantitation to ensure accuracy. The data show that this method is robust and will provide a means to obtain accurate comparative analyses of staining in IHC/IF/HC samples. |
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| Keywords: | analysis histochemistry immunofluorescence immunohistochemistry method |
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