| 药物调控对喉癌细胞系中抑癌基因表达的影响 |
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| 引用本文： | 杨静,宋岩,金明珠,季文樾. 药物调控对喉癌细胞系中抑癌基因表达的影响[J]. 中国耳鼻咽喉头颈外科, 2012, 19(7): 354-356 |
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| 作者姓名： | 杨静 宋岩 金明珠 季文樾 |
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| 作者单位： | 1.中国医科大学附属盛京医院耳鼻咽喉科,辽宁沈阳,110004;2.中国医科大学附属盛京医院耳鼻咽喉科,辽宁沈阳,110004;3.中国医科大学附属盛京医院耳鼻咽喉科,辽宁沈阳,110004;4.中国医科大学附属盛京医院耳鼻咽喉科,辽宁沈阳,110004 |
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| 摘 要： | 目的 探讨甲基化转移酶抑制剂5-氮杂-2’-脱氧胞苷(5-aza-2’-deoxycytidine,5-Aza-dC)对喉癌细胞系中抑癌基因错配修复基因(O6-methylguanine-DNA methyltransferase,MGMT)和Ras相关区域家族1A基因(ras association domain family 1A,RASSF1A)甲基化水平及基因表达的影响.方法 5-Aza-dC处理体外培养的喉癌Hep-2细胞(简称Hep-2细胞),应用实时荧光定量逆转录PCR (realtime fluro-genetic quantitative reverse transcription PCR)方法及甲基化特异性PCR (MSP)方法分别检测药物干预前后细胞中抑癌基因MGMT和RASSF1A表达及甲基化情况.结果 ①在Hep-2细胞中未经药物干预前抑癌基因MGMT和RASSF1A表现为甲基化,抑癌基因MGMT和RASSF1A弱表达.②在5-Aza-dC的作用下,Hep-2细胞系中MGMT和RASSF1A基因的甲基化状态得到了逆转,MGMT和RASSF1A基因表达上调.结论 Hep-2细胞中抑癌基因MGMT和RASSF1A启动子甲基化可能是导致其基因失活的主要原因.应用5-Aza-dC能够通过逆转抑癌基因MGMT和RASSF1A的DNA甲基化水平从而使基因表达得到了增强.
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| 关 键 词： | 喉肿瘤 细胞 培养的 DNA甲基化 基因 ras DNA错配修复 |
| 收稿时间： | 2012-02-08 |
Effect of drug regulation on tumor suppressor gene expression in Hep-2 cell line |
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| YANG Jing,SONG Yan,JIN Mingzhu,JI Wenyue. Effect of drug regulation on tumor suppressor gene expression in Hep-2 cell line[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2012, 19(7): 354-356 |
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| Authors: | YANG Jing SONG Yan JIN Mingzhu JI Wenyue |
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| Affiliation: | (Department of Otolaryngology,China Medical University Affiliated Shengjing Hospital,Shenyang,Liaoning,110004,China) |
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| Abstract: | OBJECTIVE To investigate the effect of 5-Aza-dC on tumor suppressor gene MGMT and RASSF1A expression and methylation level in Hep-2 cell line.METHODS Hep-2 cell line was cultured in vitro and treated with 5-Aza-dC.MGMT and RASSF1A expression and methylation level before and after intervention with drug were detected using realtime PCR and MSP.RESULTS ①Before intervention with drug,tumor suppressor gene MGMT and RASSF1A was weakly expressed and methylated in Hep-2 cell line.② With the effect of 5-Aza-dC,the methylation of MGMT and RASSF1A gene was reversed.The expression of MGMT and RASSF1A was enhanced.CONCLUSION In Hep-2 cell line,Promoter methylation of tumor suppressor MGMT and RASSF1A may play a very important role in loss of gene expression.5-Aza-dC can improve MGMT and RASSF1A expression by reversing DNA methylation. |
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| Keywords: | Laryngeal Neoplasms Cells Cultured DNA Methylation Genes ras DNA Mismatch Repair |
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