| miRNA-370沉默对酒精性肝病大鼠的保护作用及机制研究 |
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| 引用本文： | 辛小敏,王秀敏,黄宏春. miRNA-370沉默对酒精性肝病大鼠的保护作用及机制研究[J]. 天津医科大学学报, 2021, 0(2): 108-111 |
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| 作者姓名： | 辛小敏 王秀敏 黄宏春 |
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| 作者单位： | （安阳市人民医院消化内科,安阳 455000） |
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| 摘 要： | 目的:探究微小RNA（miRNA）-370沉默对酒精性肝病（ALD）大鼠的作用及机制。方法:32只Sprague-Dawley（SD）大鼠随机分为4组:正常组（普通饲料）、模型组（40%酒精灌胃+隔日高脂饲料饲养）、空载组（40%酒精灌胃+隔日高脂饲料饲养+尾静脉注射含空载质粒的腺病毒）和沉默组（40%酒精灌胃+隔日高脂饲料饲养+尾静脉注射含si-miRNA-370质粒的腺病毒）,每组各8只。用40%酒精灌胃联合隔日高脂饲料饲养法构建ALD大鼠模型。酶联免疫吸附法检测超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）表达水平;实时荧光定量PCR法检测miRNA-370、沉默信息调节因子6（SIRT6）mRNA和Nrf2 mRNA表达水平;蛋白质免疫印迹法检测SIRT6和Nrf2表达水平。结果:与正常组比较,模型组SOD、GSH-Px、SIRT6 mRNA、Nrf2 mRNA、SIRT6和Nrf2水平降低,丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、甘油三酯（TG）、MDA和miRNA-370水平升高,差异均有统计学意义（q=10.306、14.839、9.040、10.645、5.836、8.775、11.098、10.066、8.569、4.976、12.435,均P＜0.05）。与空载组比较,沉默组SOD、GSH-Px、SIRT6 mRNA、Nrf2 mRNA、SIRT6和Nrf2水平升高,ALT、AST、TG和miRNA-370水平降低,差异均有统计学意义（q=5.731、9.537、14.524、29.569、8.888、16.233、8.144、6.818、5.329、21.317,均P＜0.05）。结论:MiRNA-370沉默可改善ALD大鼠肝脏损伤,升高SOD和GSH-Px水平,其机制可能与调控SIRT6和Nrf2表达有关。
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| 关 键 词： | 酒精性肝病 miRNA-370 沉默信息调节因子6 核因子E2相关因子2 |
Study on the protective effect and mechanism of microRNA-370 silencing on rats with alcoholic liver disease |
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| XIN Xiao-min,WANG Xiu-min,HUANG Hong-chun. Study on the protective effect and mechanism of microRNA-370 silencing on rats with alcoholic liver disease[J]. Journal of Tianjin Medical University, 2021, 0(2): 108-111 |
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| Authors: | XIN Xiao-min WANG Xiu-min HUANG Hong-chun |
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| Affiliation: | （Department of Gastroenterology, Anyang People′s Hospital,Anyang 455000,China） |
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| Abstract: | Objective: To explore the effect and mechanism of microRNA（miRNA）-370 silencing on rats with alcoholic liver disease （ALD）. Methods:Thirty-two Sprague-Dawley（SD）rats were randomly divided into 4 groups: normal group （common feed）,model group （40% alcohol gavage + high-fat feed feeding every other day）,no-load group（40% alcohol gavage + high-fat feed feeding every other day + tail vein injection of adenovirus containing empty plasmid） and silent group（40% alcohol gavage + high-fat feed feeding every other day + tail vein injection of adenovirus containing si-miRNA-370 plasmid）,8 rats in each group. An ALD rat model was constructed using 40% alcohol gavage combined with high-fat feed every other day. Enzyme-linked immunosorbent assay was used to detect the expression levels of superoxide dismutase （SOD）,glutathione peroxidase（GSH-Px） and malondialdehyde（MDA）. Real-time fluorescence quantitative PCR method was used to detect miRNA-370 and SIRT6 mRNA and Nrf2 mRNA expression levels. Western blotting was used to detect the expression levels of SIRT6 and Nrf2. Results:Compared with the normal group,the levels of SOD,GSH-Px,SIRT6 mRNA,Nrf2 mRNA,SIRT6 and Nrf2 in the model group were decreased,and the levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,triglyceride（TG）,MDA and miRNA-370 were increased,the differences were statistically significant （q=10.306, 14.839,9.040,10.645,5.836,8.775,11.098,10.066,8.569,4.976,12.435,all P<0.05）. Compared with the no-load group,the levels of SOD,GSH-Px,SIRT6 mRNA,Nrf2 mRNA,SIRT6,and Nrf2 in the silent group increased,while the levels of ALT,AST,TG and miRNA-370 decreased,the differences were statistically significant （q=5.731,9.537,14.524,29.569,8.888,16.233,8.144,6.818,5.329, |
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| Keywords: | alcoholic liver disease microRNA-370 silent information regulator 6 nuclear factor E2 related factor 2 |
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