首页 | 本学科首页   官方微博 | 高级检索  
     


Localization of a T-cell epitope within the nucleocapsid protein of avian coronavirus.
Authors:A M Boots   J G Kusters   J M van Noort   K A Zwaagstra   E Rijke   B A van der Zeijst     E J Hensen
Affiliation:Institute of Molecular Biology and Medical Biotechnology, University of Utrecht, The Netherlands.
Abstract:In a previous study, two murine T-cell hybridomas generated after immunization with infectious bronchitis virus (IBV) were shown to be responsive to the internally localized viral nucleocapsid protein. In the present study, the antigenic determinants were mapped using recombinant expression products and synthetic peptides. Both hybridomas recognized the region spanning amino acid residues 71 to 78 of the nucleocapsid protein. The experimentally determined epitope corresponded with predicted motifs. Both an I-Ed binding motif and a predicted cleavage site for the aspartyl protease cathepsin D were contained within the sequence. The epitope was shown to prime cellular immune responses to IBV in the chicken.
Keywords:
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号