| 氧化剂致CHL细胞DNA损伤及酪醇的保护作用 |
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| 引用本文： | 李明正, 金中初. 氧化剂致CHL细胞DNA损伤及酪醇的保护作用[J]. 中国公共卫生, 2002, 18(7): 776-778. DOI: 10.11847/zgggws2002-18-07-05 |
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| 作者姓名： | 李明正 金中初 |
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| 作者单位： | 1.空军杭州疗养院康复疗养科 杭州310013;;2.浙江大学医学院病理生理学教研室 |
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| 基金项目： | 国家自然科学基金资助项目(No 39970649) |
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| 摘 要： | 目的 检测阿霉素、重铬酸钾和过氧化氢所致中国仓鼠肺成纤维细胞(CHL)DNA的损伤效应及酪醇对真核细胞DNA氧化损伤的保护作用.方法 CHL细胞用不同浓度化学物作用一定时间后(阿霉素:1.25h;重铬酸钾:1.75h;过氧化氢:2.5min),收获细胞用单细胞凝胶电泳技术检测DNA链断裂情况;CHL细胞先用不同浓度酪醇作用1.58h,再加入0.4mmol/L过氧化氢共同培养25min,然后用单细胞凝胶电泳技术测定DNA链断裂情况,分析酪醇的抗氧化损伤作用.结果 0.1μmol/L阿霉素、0.1mmol/L重铬酸钾及0.1mmol/L过氧化氢作用一定时间后,均可引起DNA链断裂,随着各组处理物浓度的增加,DNA迁移长度和拖尾细胞百分率也相应增加,两者剂量反应关系明显.10μg/ml、20μg/ml酪醇的预处理可使过氧化氢染毒细胞的拖尾百分率和DNA迁移长度显着降低.结论 阿霉素、重铬酸钾和过氧化氢均是强烈的DNA链断裂剂;酪醇对真核细胞DNA氧化损伤具有抑制作用.
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| 关 键 词： | 单细胞凝胶电泳/彗星试验 DNA氧化损伤/DNA链断裂 酪醇 抗氧化 |
| 文章编号： | 1001-0580(2002)07-0776-03 |
| 收稿时间： | 2001-03-19 |
| 修稿时间： | 2001-03-19 |
DNA Oxidative Damage Induced by Oxidants and P-tyrosol Protection Against Hydrogen Peroxide in CHL Cells |
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| LI Ming-zheng, JIN Zhong-chu. DNA Oxidative Damage Induced by Oxidants and P-tyrosol Protection Againsthydrogen Peroxide in CHL Cells[J]. Chinese Journal of Public Health, 2002, 18(7): 776-778. DOI: 10.11847/zgggws2002-18-07-05 |
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| Authors: | LI Ming-zheng JIN Zhong-chu |
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| Affiliation: | 1.Department of Pathophysiology, College of Medicine, Zhejiang University, Hangzhou 310013, China |
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| Abstract: | Objective To detect the DNA strand breaks induced by adriamycin,potassium chromate or hydrogen peroxide and to study the effect of p-tyrosol on DNA oxidative damage in CHL cells.Methods Single cell gel electrophoresis assay(SCGE)was used to detect DNA strand breaks when CHL cells were induced by different concentration of compounds for certain time(adriamycin:1.25h,potassium chromate:1.75h,hydrogen peroxide:25min);SCGE was used to detect DNA strand breaks and to analyze p-tyrosol protection against oxidative damage in CHL cells which were exposed to different concentration of p-tyrosol for 1.58h firstly,and then cultured with 014mmol/L hydrogen peroxide for 25min.Results Adriamycin,potassium chromate or hydrogen peroxide tested at all concentrations,caused a significant increase in DNA migration measured as tail length and percentage of cells with migrated DNA in exposed CHL cells,and the dose-response relationships are apparent between the tail length and the concentration of compound.The pretreatment of p-tyrosol(10μg/ml or 20μg/ml)markedly decreased the tail length of CHL cells treated by hydrogen peroxide.Conclusion Adriamycin,potassium chromate and hydrogen peroxide are violent DNA breaking agent.P-tyrosol has the ability to inhibit oxidative DNA damage in eukaryote. |
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| Keywords: | single cell gel electrophoresis/comet assay DNA oxidative damage/DNA strand breaks p-tyrosol antioxidation |
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