| 反相高效液相色谱法定量分析二甲胺四环素 |
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| 引用本文： | 陈桂良, 陈从恒, 蒋建兰. 反相高效液相色谱法定量分析二甲胺四环素[J]. 中国现代应用药学, 1994, (1): 33-35. |
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| 作者姓名： | 陈桂良,陈从恒 蒋建兰 |
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| 作者单位： | 上海市药品检验所(陈桂良，陈从恒)，上海市环境保护科学研究所(蒋建兰) |
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| 摘 要： | 利用反相高效液相色谱法测定二甲胺四环素含量，该法使用２００×５ｍｍ内径的色谱柱，内装ＹＷＧ－Ｃ１８Ｈ３７。，柱温２５±０．５℃，流动相为０．００２ｍｏｌ／Ｌ ＥＤＴＡ－Ｎａ２溶液（用草酸调节ｐＨ３．０±０．１）：甲醇：乙腈（３：１：１Ｖ／Ｖ／Ｖ），流速为１ｍｌ／ｍｉｎ，紫外检测波长为２８０ｕｍ，测定了１５批国内外厂家生产的二甲胺四环素联囊的含量，结果与用美国药典（２２版）法所测结果一致。
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| 关 键 词： | 二甲胺四环素 反相高效液相色谱法 |
Quantitative Analysis of Minocycline with RP-HPLC |
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| Chen Guiliang, Chen Congheng, Jiang Jianlan. Quantitative Analysis of Minocycline with RP-HPLC[J]. Chinese Journal of Modern Applied Pharmacy, 1994, (1): 33-35. |
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| Authors: | Chen Guiliang Chen Congheng Jiang Jianlan |
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| Abstract: | A RP-HPLC method was established for analysing minocycline. The HPLC conditionswere:200 5 mm ID column containing YWG-C18H37, held at 25+0.5C mobile phase of 0.002mol/L EDTA-Na2 buffer solution (adjust PH=3.0 +0.1 with oxalic acid): meethanol: acetonitrile(3:1:1 v/v/v) at a flow rate of 1 ml/min; the column effluent monitored at 280 um. Fifteenbatones of minocycline capsules produced ill domestic and foreign pharmaceutical factories weredetermined, and the results were the same as those oh tamed by USPXXll method. |
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| Keywords: | Minocycline RP-HPLC |
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