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| 阿司匹林对S180肿瘤生长及血管生长相关因子表达的抑制作用 | |
| 引用本文： | 高雪芹,张维东,宋守芹,武利存,王丽. 阿司匹林对S180肿瘤生长及血管生长相关因子表达的抑制作用[J]. 中国药理学与毒理学杂志, 2002, 16(4): 299-301 |
| 作者姓名： | 高雪芹 张维东 宋守芹 武利存 王丽 |
| 作者单位： | 山东省医学科学院基础医学研究所,山东济南,250062 |
| 摘 要： | 目的 从抗血管生成的角度研究阿司匹林(Asp)预防和治疗肿瘤的机理。方法 昆明种小鼠随机分为对照组 ,替加氟组 ,Asp 5 0 ,2 5 ,10mg·kg- 1组 ,于接种S180肿瘤细胞后d 2开始ig给药 ,连续9d ,观察抑瘤率。采用免疫组化的方法研究Asp对肿瘤组织环氧合酶 2 (COX 2 )及血管相关生长因子的作用。结果 Asp 3个剂量组均有一定的抑瘤作用 ,大剂量抑瘤率为 2 1.1%。免疫组化染色显示Asp对肿瘤组织的COX 2表达有明显的抑制作用 ,同时血管生长相关因子血管内皮生长因子、纤维细胞生长因子 2表达也明显下调 ,肿瘤组织微血管密度明显降低。结论 Asp对S180肿瘤有抑制作用 ,它可以抑制与血管生长相关的COX 2的表达而抑制肿瘤血管的生长 ,进而抑制肿瘤的生长。抑制肿瘤血管的生长可能是Asp预防和治疗肿瘤的机理之一。 |
| 关 键 词： | 阿司匹林 细胞,S180肉瘤 前列腺素内过氧化物合酶 物质,内皮生长,血管 成纤维细胞生长因子,碱性 |
| 收稿时间： | 2001-10-29 |
Inhibitory effects of aspirin on the transplanted sarcoma S180 and the expression of angiogenesis related factors |
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| GAO Xue-Qin, ZHANG Wei-Dong, SONG Shou-Qin, WU Li-Cun, WANG Li. Inhibitory effects of aspirin on the transplanted sarcoma S180 and the expression of angiogenesis related factors[J]. Chinese Journal of Pharmacology and Toxicology, 2002, 16(4): 299-301 | |
| Authors: | GAO Xue-Qin ZHANG Wei-Dong SONG Shou-Qin WU Li-Cun WANG Li |
| Affiliation: | (Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062, China) |
| Abstract: | AIM To investigate the antiangiogenesis by which aspirin exterts its tumor chemopreventive and chemotherapeutic effects. METHODS Kunming mice were randomizedly divided into control group, tegafur (3 mg per mouse) positive group and aspirin 50, 25, 10 mg·kg-1 groups. One day after inoculation of 0.2 mL S180 cell(2.5×1010 L-1) suspension, tegafur and aspirin was given by gastric intubation for 9 d. The inhibition rate on S180 was calculated routinely. The expression of cyclooxygenase-2(COX-2), vascular endothelial growth factor(VEGF), fibroblast growth factor-2(FGF-2) and microvassel density(MVD) was detected by immunohistochemical staining. RESULTS The growth of sarcoma S180 was significantly inhibited by aspirin at the dose of 50 mg·kg-1, with the inhibitory rate 21.1%. The expression of COX- 2 in the tumor tissue was also inhibited by aspirin. And accordingly the expression of VEGF, FGF-2 and MVD was also markedly inhibited dose-dependently by aspirin. CONCLUSION Aspirin has inhibitory effects on sarcoma S180, and it decreases the expression of COX-2 in tumor tissue. There has positive relation between the expression of COX- 2 and angiogenesis related factor. Antiangiogenesis may be one of mechanisms by which aspirin exerts its tumor chemopreventive and therapeutic effects. |
| Keywords: | aspirin cells sarcoma 180 prostaglandin endoperoxide synthase substance endothelial growth vascular fibroblast growth factor basic |
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