Effects of Nano‐hydroxyapatite/Poly(DL‐lactic‐co‐glycolic acid) Microsphere‐Based Composite Scaffolds on Repair of Bone Defects: Evaluating the Role of Nano‐hydroxyapatite Content |
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| Authors: | Shu He Kai‐Feng Lin Zhen Sun Yue Song Yi‐Nan Zhao Zheng Wang Long Bi Jian Liu |
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| Affiliation: | Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an, China |
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| Abstract: | The aim of the current study was to prepare microsphere‐based composite scaffolds made of nano‐hydroxyapatite (nHA)/poly (DL‐lactic‐co‐glycolic acid) (PLGA) at different ratios and evaluate the effects of nHA on the characteristics of scaffolds for tissue engineering application. First, microsphere‐based composite scaffolds made of two ratios of nHA/PLGA (nHA/PLGA = 20/80 and nHA/PLGA = 50/50) were prepared. Then, the effects of nHA on the wettability, mechanical strength, and degradation of scaffolds were investigated. Second, the biocompatibility and osteoinductivity were evaluated and compared by co‐culture of scaffolds with bone marrow stromal stem cells (BMSCs). The results showed that the adhesion, proliferation, and osteogenic differentiation of BMSCs with nHA/PLGA (50/50) were better than those with nHA/PLGA (20/80). Finally, we implanted the scaffolds into femur bone defects in a rabbit model, then the capacity of guiding bone regeneration as well as the in vivo degradation were observed by micro‐CT and histological examinations. After 4 weeks' implantation, there was no significant difference on the repair of bone defects. However, after 8 and 12 weeks' implantation, the nHA/PLGA (20/80) exhibited better bone formation than nHA/PLGA (50/50). These results suggested that a proper concentration of nHA in the nHA/PLGA composite should be taken into account when the composite scaffolds were prepared, which plays an important role in the biocompatibility, degradation rate and osteoconductivity. |
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| Keywords: | Nano‐hydroxyapatite Poly(DL‐lactic‐co‐glycolic acid) Microsphere‐based composite Bone tissue engineering Bone regeneration |
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