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| 代森锰锌对PC-12细胞凋亡的影响 | |
| 引用本文： | 张劲松,栾春业,王强,徐艳,张晶,环飞,程洁,肖杭. 代森锰锌对PC-12细胞凋亡的影响[J]. 中国药理学与毒理学杂志, 2011, 25(4): 349-353. DOI: 10.3867/j.issn.1000-3002.2011.04.004 |
| 作者姓名： | 张劲松 栾春业 王强 徐艳 张晶 环飞 程洁 肖杭 |
| 作者单位： | 1. 南京医科大学第一附属医院急诊中心,江苏,南京,210029 2. 滨州医学院附属医院急诊科,山东滨州,256603 3. 南京医科大学公共卫生学院神经毒理研究室,江苏,南京,210029 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的探讨代森锰锌对PC-12细胞凋亡的影响及其机制。方法 采用体外细胞培养方法,PC-12细胞加入代森锰锌0,1,10,30,60和120μmol.L-1,培养24h后,应用WST-8法检测PC-12细胞增殖;PC-12细胞中加入代森锰锌0,1,30和120μmol.L-1,培养24h后,流式细胞术FITC-AnnexinⅤ/PI双染检测细胞凋亡率;Hoechst33258染色及倒置荧光显微镜观察细胞形态学改变;Western印迹法检测Bcl-2和Bax的表达以及ERK蛋白磷酸化水平。结果与正常对照组相比,随着代森锰锌浓度增加,代森锰锌组晚期凋亡率升高,呈浓度依赖关系,IC50为49.95μmol.L-1。代森锰锌120μmol.L-1组细胞晚期凋亡率为(90±4)%(P<0.05);Hoechst33258染色可见细胞核膨大、染色质边集浓染等凋亡特征;与正常对照组相比,Bcl-2逐渐降低,Bax和p-ERK1/2表达增高(P<0.05),代森锰锌120μmol.L-1组p-ERK1/2积分吸光度分别为128.0±2.5和178.4±4.0。结论代森锰锌能够诱导PC-12细胞凋亡,ERK信号通路可能在此过程中发挥作用。 |
| 关 键 词： | 内分泌干扰物 代森锰锌 细胞凋亡 |
| 收稿时间： | 2010-11-10 |
Effects of mancozeb on apoptosis of PC-12 cells |
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| ZHANG Jin-song,LUAN Chun-ye,WANG Qiang,XU Yan,ZHANG Jing,HUAN Fei,CHENG Jie,XIAO Hang. Effects of mancozeb on apoptosis of PC-12 cells[J]. Chinese Journal of Pharmacology and Toxicology, 2011, 25(4): 349-353. DOI: 10.3867/j.issn.1000-3002.2011.04.004 | |
| Authors: | ZHANG Jin-song LUAN Chun-ye WANG Qiang XU Yan ZHANG Jing HUAN Fei CHENG Jie XIAO Hang |
| Affiliation: | (1. Department of Emergency, the First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China; 2. Department of Emergency, affiliated Hospital, Binzhou Medical University, Binzhou 56603; 3. Laboratory of Neurotoxicology, School of Public Health, Nanjing Medical niversity, Nanjing 210029, China) |
| Abstract: | OBJECTIVE To investigate the effect of mancozeb on the apoptosis of PC-12 cells. METHODS The PC-12 cells were cultured for 24 h after mancozeb 0, 1, 10, 30, 60 and 120 μmol·L-1 were added. Cell Counting Kit-8 was used to assess the proliferation and toxicity induced by mancozeb. Twenty-four hours after mancozeb 0, 50 μmol·L-1 was added to PC-12 cells, the morphological changes of PC-12 cells were observed by a microscope, and cell apoptosis rates were detected by FITC-AnnexinⅤ/PI flow cytometry. The expression of Bcl-2, Bax and p- ERK1/2 was determined by Western blot. RESULTS Compared with normal control group, PC-12 cells in mancozeb groups showed higher apoptosis rates and evident morphological changes that became more evident with the dose of mancozeb. The IC50 was 49.95 μmol·L-1, the apoptosis rate of PC-12 cells in mancozeb 120 μmol·L-1 group was (90±4)％. The Bax protein levels increased and the Bcl- 2 protein levels in mancozeb groups were decreased. Compared with normal control group, the p-ERK1/2 expression was significantly up-regulated. The grey value of p-ERK1/2 in mancozeb 120 μmol·L-1 group was 128.0±2.5 and 178.4±4.0. CONCLUSION Mancozeb can induce apoptosis of PC-12 cells, in which the expression of p-ERK1/ 2 proteins may play a role in PC-12 apoptosis. |
| Keywords: | endocrine disruptors mancozeb apoptosis |
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