| 万胜化风丹、雄黄和朱砂的急性肝肾毒性作用 |
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| 引用本文： | 颜俊文,苗加伟,何海洋,时京珍,吴芹,刘杰,石京山. 万胜化风丹、雄黄和朱砂的急性肝肾毒性作用[J]. 中国药理学与毒理学杂志, 2011, 25(4): 380-385. DOI: 10.3867/j.issn.1000-3002.2011.04.010 |
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| 作者姓名： | 颜俊文 苗加伟 何海洋 时京珍 吴芹 刘杰 石京山 |
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| 作者单位： | 1. 贵州省基础药理重点实验室,遵义医学院药理教研室,贵州,遵义,563003
2. 贵阳中医学院实验中心基础医学实验室,贵州,贵阳,550002 |
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| 基金项目： | 贵州省中药现代化科技产业研究开发专项资助项目 |
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| 摘 要： | 目的研究万胜化风丹中雄黄和朱砂的肝肾毒性作用,探讨目前对其毒性评价指标的合理性。方法 成年昆明种小鼠分别一次性ig给予万胜化风丹(原方药)3g.kg-1、雄黄和朱砂减量的万胜化风丹(减量方药)3g.kg-1、不含雄黄和朱砂的万胜化风丹(减方药)3g.kg-1、雄黄0.3g.kg-1、朱砂0.3g.kg-1、亚砷酸钠36mg.kg-1和氯化汞0.07g.kg-1,8h后检测肝及肾组织中砷和汞的含量,检测血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、肌酐(Cre)、尿素氮(BUN)含量;RT-PCR方法检测肝和肾中金属硫蛋白基因(MT-1)的表达。结果亚砷酸钠、原方药及减量方药组肝和肾组织中砷的蓄积量明显增加(P<0.05),且亚砷酸钠>原方药>减量方药。亚砷酸钠组ALT显著升高,其他各组略有升高,但与正常对照组无显著差异。氯化汞和朱砂组肝肾组织中汞的蓄积量明显增加(P<0.05),且氯化汞组>朱砂,氯化汞组同时伴Cre、BUN显著升高(P<0.05)。亚砷酸钠组、氯化汞组肝肾病理损伤明显,MT-1mRNA在肝肾组织的高表达。结论万胜化风丹、雄黄和朱砂的急性肝肾毒性远低于亚砷酸钠和氯化汞。
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| 关 键 词： | 万胜化风丹 雄黄 朱砂 亚砷酸钠 氯化汞 急性毒性 |
| 收稿时间： | 2010-10-28 |
Acute hepatic and renal toxicy of Wansheng Huafeng Dan,realgar and cinnabar |
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| YAN Jun-wen,MIAO Jia-wei,HE Hai-yang,SHI Jing-zheng,WU Qin,LIU Jie,SHI Jing-shan. Acute hepatic and renal toxicy of Wansheng Huafeng Dan,realgar and cinnabar[J]. Chinese Journal of Pharmacology and Toxicology, 2011, 25(4): 380-385. DOI: 10.3867/j.issn.1000-3002.2011.04.010 |
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| Authors: | YAN Jun-wen MIAO Jia-wei HE Hai-yang SHI Jing-zheng WU Qin LIU Jie SHI Jing-shan |
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| Affiliation: | (1. Department of Pharmacology, Zunyi Medical College, Zunyi 563003, China; 2. Basic Medical Laboratory of Experimental Center, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China) |
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| Abstract: | OBJECTIVE To explore the hepatic and renal toxicy of realgar and cinnabar in Wansheng Huafeng Dan (WSHFD). METHODS Mice were ig given WSHFD 3 g·kg-1 that contained 0%, 3% or 10% realgar and cinnabar respectively, realgar 0.3 g·kg-1, cinnabar 0.3 g·kg-1, NaAsO2 0.036 g·kg-1 and HgCl2 0.07 g·kg-1. Eight hours later, As and Hg contents in liver and kidneys, alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine (Cre), histopathology, and metallothionein 1 mRNA(MT-mRNA) expression were determined. RESULTS Accumulation of As in liver and kidneys in WSHFD containing 10% or 3% realgar and cinnabar groups and NaAsO2 group was significantly higher than that in normal control group(P<0.05). Accumulation of Hg in kidneys in cinnabar and HgCl2 groups was significantly higher than in WSHFD groups containing 10% realgar and cinnabar (P<0.05). Compared with normal control group and WSHFD groups containing 10% realgar and cinnabar, ALT activity significantly increased in NaAsO2 group, and Cre and BUN significantly increased in HgCl2 group. Compared with WSHFD groups containing 10% reaglar and cinnabar, the liver and kidneys in NaASO2 and HgCl2 groups were injured and MT-1 mRNA expression was upregulated (P<0.05). CONCLUSION Acute toxicity of realgar, cinnabar, and WSHFD is far below those of NaAsO2 and HgCl2. |
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| Keywords: | Wansheng Huasheng Dan realgar cinnabar arsenite mercuric chloride acute toxicity |
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