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| HSV-2 LAT过表达vero细胞中microRNA的表达谱变化 | |
| 引用本文： | 王颖,樊建勇,杨慧兰,陈剑云. HSV-2 LAT过表达vero细胞中microRNA的表达谱变化[J]. 南方医科大学学报, 2012, 32(10): 1440-1444 |
| 作者姓名： | 王颖 樊建勇 杨慧兰 陈剑云 |
| 作者单位： | 1. 广州军区广州总医院皮肤科,广东 广州,510010 2. 福建省73881部队卫生处,福建 福州,350003 |
| 基金项目： | 国家自然科学基金，广东省科技计划 |
| 摘 要： | 目的分析过表达疱疹病毒Ⅱ型（HSV-2）潜伏相关转录子LAT后vero细胞microRNA表达谱的表达变化。方法通过化 学合成的方法获得LAT基因的全长序列。构建HSV-2LAT逆转录病毒表达载体pRetroQ- AcGFP1-C1，包装获得HSV-2LAT 基因过表达的逆转录病毒。利用microRNA芯片技术，分析感染逆转录病毒HSV-2LAT后vero细胞microRNA表达谱的变化， 得到因LAT基因过表达而产生变化的microRNA。结果通过microRNA芯片分析，逆转录病毒HSV-2LAT感染vero细胞后， 可引起hsa-miR-23a*、kshv-miR-K12-3、hsa-miR-943、hsa-miR-634、hsa-miR-12705种microRNA2倍以上上调；使hsa-miR-181a-2*、hsa-miR-450b-5p、hsa-miR-31、hsa-miR-24、kshv-miR-K12-12*5种microRNA2倍以上下调。结论LAT基因过表达后 可引起vero细胞microRNA表达谱的变化。 |
| 关 键 词： | 疱疹病毒Ⅱ型 LAT microRNA 基因芯片 逆转录病毒 |
Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression |
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| WANG Ying , FAN Jianyong , YANG Huilan , CHEN Jianyun. Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression[J]. Journal of Southern Medical University, 2012, 32(10): 1440-1444 | |
| Authors: | WANG Ying FAN Jianyong YANG Huilan CHEN Jianyun |
| Affiliation: | 1Department of Dermatology,Guangzhou General Hospital of Guangzhou Military Command,Guangzhou 510010,China;2Medical Service Division,73881 Unit of PLA,Fuzhou 350003,China |
| Abstract: | Objective To investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2 LAT overexpression.Methods The full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ-AcGFP1-C1 vector,and the recombinant retrovirus expressing HSV-2 LAT was packaged.Using a microRNA microarray,the microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.Results In Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression,5 microRNAs(hsa-miR-23a*,kshv-miR-K12-3,hsa-miR-943,hsa-miR-634,and hsa-miR-1270) were up-regulated and 5(hsa-miR-181a-2*,hsa-miR-450b-5p,hsa-miR-31,hsa-miR-24,and kshv-miR-K12-12*) were down-regulated.Conclusion The expression of HSV-2 LAT can induce changes in microRNA expression profile in Vero cells. |
| Keywords: | herpes simplex virus type 2 LAT microRNA microarray retrovirus |
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