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Challenges in the identification of methicillin‐resistant Staphylococcus argenteus by routine diagnostics |
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| Authors: | Hege Smith Tunsjø Sumana Kalyanasundaram Colin Charnock Truls Michael Leegaard Aina E. F. Moen |
| Affiliation: | 1. Department of Health Sciences, OsloMet – Oslo Metropolitan University, Oslo, Norway;2. Department of Microbiology and Infection Control, Akershus University Hospital, L?renskog, Norway;3. Department of Core Facilities, Bioinformatics Core Facility, Institute of Cancer Research, Radium Hospital, Oslo University Hospital, Oslo, Norway;4. Department of Clinical Molecular Biology (EpiGen), Akershus University Hospital, L?renskog, Norway;5. Institute of Clinical Medicine, University of Oslo, Oslo, Norway |
| Abstract: | Current clinical diagnostic procedures have shortcomings in the differentiation of Staphylococcus argenteus from Staphylococcus aureus. This article presents three cases of Staphylococcus argenteus obtained from clinical samples. The initial results from biochemical and molecular methods led to an incorrect identification of the isolates as methicillin‐resistant Staphylococcus aureus. Whole genome sequencing and real‐time PCR targeting the nonribosomal peptide synthetase gene led to their correct identification as methicillin‐resistant Staphylococcus argenteus. The study shows that real‐time PCR can be used to differentiate the two species in routine diagnostics. For purposes of identification based on whole genome sequencing, the MinION portable sequencer is a simple and affordable approach which could be used by many laboratories. |
| Keywords: | MinION sequencing rapid diagnostic methods Staphylococcus argenteus Staphylococcus aureus whole genome sequencing |