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| 八氯代二苯并二噁英对小鼠睾丸组织基因表达谱的影响 | |
| 引用本文： | 张伟,王耀峰,倪天军,杨志军. 八氯代二苯并二噁英对小鼠睾丸组织基因表达谱的影响[J]. 南方医科大学学报, 2016, 36(3): 316-320. DOI: 10.3969/j.issn.1673-4254.2016.03.04 |
| 作者姓名： | 张伟 王耀峰 倪天军 杨志军 |
| 作者单位： | 1. 新乡医学院基础医学院,河南 新乡,453003;2. 新乡医学院基础医学院，河南 新乡 453003; 新乡医学院三全学院，河南 新乡 453003 |
| 基金项目： | 国家自然科学基金(81401470)；河南省教育厅自然科学研究计划(13B150216；14B150023)；新乡医学院高学历人才启动基金(505001)Supported by National Natural Science Foundation of China (81401470) |
| 摘 要： | 目的探讨八氯代二苯并二噁英（OCDD）对雄性小鼠睾丸基因表达谱改变的影响。方法20只雄性CBL/6j小鼠，随机分 为对照组（玉米油）、OCDD低剂量组（1.25×10-6 g/mL）、中剂量组（1.25×10-5 g/mL）和高剂量组（1.25×10-4 g/mL），每天灌胃1次， 连续灌胃30 d后睾丸取材，应用基因芯片技术比较不同浓度OCDD染毒组和对照组雄性小鼠睾丸基因表达差异。结果与对照 组相比，低剂量组和对照组差异基因总数1 133个，上调659个，下调474个；中剂量组和对照组差异基因数978个，上调487个， 下调491个；高剂量组和对照组的差异基因数895个，上调424个，下调471个。结论亚慢性OCDD暴露后对小鼠的睾丸相关基 因表达造成影响，提示睾丸可能是OCDD 毒性作用的靶器官。 |
| 关 键 词： | 八氯代二苯并二噁英 睾丸 基因表达 基因芯片 |
Effect of 1, 2, 3, 4, 6, 7, 8, 9-octachlorodibenzo-p-dioxin on testicular gene expression profiles in male mice |
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| Abstract: | Objective To investigate the effect of 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin (OCDD) on the testicular gene expression profile in the testis of mice. Methods Twenty male C57BL/6j mice were randomly divided into normal control group (fed with maize oil) and 3 OCDD groups treated with OCDD by gavage for 30 days at low-, moderate-, and high doses of 1.25×10-6, 1.25 ×10-5, and 1.25 ×10-4 g/mL, respectively (8 mL/kg daily). The testicular gene expression profiles of the mice were investigated using gene chip technique and compared between OCDD-exposed groups and the control group. Results Compared with the control group, the mice in low-dose OCDD group showed 1133 differentially expressed genes, including 659 up-regulated and 474 down-regulated ones;in the moderate-dose OCDD group, 978 genes were differentially expressed, including 487 up-regulated and 491 down-regulated ones; in the high-dose group, 895 genes were differentially expressed, including 424 up-regulated and 471 down-regulated ones. Conclusion The effect of sub-chronic exposure to OCDD on testicular gene expression profiles in male C57BL/6j mice indicates that the testis is probably the target organ of OCDD. |
| Keywords: | 1,2,3,4,6,7,8,9-octachlorodibenzo-p-dioxin testis gene expression gene chip |
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