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| 人 G类有机磷毒剂水解酶的性质(英文) | |
| 引用本文： | 陈惠琼,张翰,杜先林,孙曼霁. 人 G类有机磷毒剂水解酶的性质(英文)[J]. 中国药理学与毒理学杂志, 2000, 14(2): 113-115 |
| 作者姓名： | 陈惠琼 张翰 杜先林 孙曼霁 |
| 作者单位： | (军事医学科学院毒物药物研究所， 北京 100850) |
| 摘 要： | 观察了部分纯化的人肝 G类毒剂水解酶 ( G酶 )的一些生化性质 .二硫苏糖醇 ( 5mmol·L-1)使G酶活性在 37℃ ,30 min内抑制 35% ;对氯汞苯甲酸 ( 1 - 1 0 0 0 μmol· L-1)不影响 G酶活性 .说明二硫键对酶分子的三维结构至关重要 ,而没有游离巯基参与酶的催化反应 .人肝 G酶专一性地水解带P- F键的有机磷化合物梭曼 ,但不催化带 P- O,P- C或 P- S键的有机磷化合物的反应 . |
| 关 键 词： | 有机磷化合物 化学战剂 神经性毒剂 杀虫剂 水解酶类 |
| 收稿时间： | 1999-06-04 |
Characteristics of human G-type organophosphate hydrolyzing enzyme |
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CHEN Hui-Qiong, ZHANG Han, DU Xian-Lin, SUN Man-Ji . Characteristics of human G-type organophosphate hydrolyzing enzyme[J]. Chinese Journal of Pharmacology and Toxicology, 2000, 14(2): 113-115 |
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| Authors: | CHEN Hui-Qiong ZHANG Han DU Xian-Lin SUN Man-Ji |
| Affiliation: | (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China) |
| Abstract: | The biochemical properties of a partially purified human liver G- type organophosphate hydrolyzing enzyme (Gase) were examined. The enzyme activity was inhibited 35% by dithiothreitol (5 mmol·L-1) at 37℃ in 30 min, however no inhibitory effect was produced by p-chloromercaptobenzoic acid (1-1000 μmol·L-1). It suggests that the disulfide bond(s) be essential for the three- dimentional structure of the Gase molecule, and there be no free sulfhydryl group taking part in the catalytic reaction. Human liver Gase specifically hydrolyzed organophosphorus compounds bearing P-F bond (soman), however it did not catalyze the hydrolysis of compounds with P-O, P-C or P-S bonding. |
| Keywords: | organophosphorus compounds chemical warfare agents nerve agents pesticides hydrolases |
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