| 甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响 |
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| 引用本文： | 王吉锡,邓伟生,王凤儒,史之茂,孟丹,徐强,张玉瑶. 甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响[J]. 实用肿瘤学杂志, 2017, 31(5): 390-395. DOI: 10.11904/j.issn.1002-3070.2017.05.002 |
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| 作者姓名： | 王吉锡 邓伟生 王凤儒 史之茂 孟丹 徐强 张玉瑶 |
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| 作者单位： | 黑龙江中医药大学(哈尔滨 150040) |
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| 基金项目： | 黑龙江省教育厅科学技术研究项目(12541764) |
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| 摘 要： | 目的 研究甘草提取物的有效部位(GL)体外诱导HeLa细胞凋亡情况及对Caspase家族蛋白表达的调控,进一步阐明GL的抑癌机制。方法 将25 μg/mL的GL作用于HeLa细胞24 h后,采取MTT比色法,吖啶橙/溴乙锭荧光双染法,透射电子显微镜法,观察GL诱导HeLa细胞凋亡情况,并采用Western blot法检测Caspase-3、Caspase-9蛋白表达水平的变化。结果 MTT结果显示,GL可以有效抑制HeLa细胞的增殖,呈剂量-时间相关性。AO/EB双染色及透射电镜观察发现GL作用HeLa细胞24 h后,可见大量早期凋亡细胞。Western blot测定结果显示,药物组Pro-caspase-9蛋白表达量与对照组比较差异具有统计学意义(P＜0.05);Cleaved-caspase-3蛋白表达量高于对照组。结论 GL能够促进Caspase-3、Caspase-9酶原活化,具有体外诱导HeLa细胞凋亡的作用。
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| 关 键 词： | 甘草 抗肿瘤作用 细胞凋亡 HeLa细胞 凋亡蛋白 |
| 收稿时间： | 2017-02-09 |
Active part of Glycyrrhiza extract induces apoptosis in human cervica l cancer HeLa cells and its related protein caspase-3 and caspase-9 expression |
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| WANG Jixi,DENG Weisheng,WANG Fengru,SHI Zhimao,MENG Dan,XU Qiang,ZHANG Yuyao. Active part of Glycyrrhiza extract induces apoptosis in human cervica l cancer HeLa cells and its related protein caspase-3 and caspase-9 expression[J]. Journal of Practical Oncology, 2017, 31(5): 390-395. DOI: 10.11904/j.issn.1002-3070.2017.05.002 |
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| Authors: | WANG Jixi DENG Weisheng WANG Fengru SHI Zhimao MENG Dan XU Qiang ZHANG Yuyao |
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| Affiliation: | Heilongjiang University of Chinese Medicine,Harbin 150040,China |
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| Abstract: | Objective The aim of this study was to determine whether active part of Glycyrrhiza extract (GL)induced apoptosis in Hela cells and its inhibitory mechanism.Methods HeLa cells were treated with 25 g/mL of GL for 24hs.Cell viability and apoptosis in HeLa cells were determined by MTT,AO/EB fluorescent double staining,transmission electron microscope(TEM),and Western blot.Results The MTT results showed that GL significantly inhibited the proliferation of HeLa cells in a dose-response.After treatment for 24 hrs,large number of early apoptotitc cell were observed using AO/EB fluorescent double staining and TEM.The expression of Pro-caspase-9 and Cleaved-caspase-3 protein was higher in GL-treated cells them those of the control cells(P<0.05).Conclusion GL can activate Caspase-3 and Caspase-9 genes to induce apoptosis in HeLa cells. |
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| Keywords: | Licorice Anti-tumor effect Apoptosis HeLa cells Apoptosis protein |
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