| 积雪草甙、羟基积雪草甙对体外培养人成纤维细胞增殖及胶原合成的影响 |
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| 引用本文： | 章庆国,赵士芳. 积雪草甙、羟基积雪草甙对体外培养人成纤维细胞增殖及胶原合成的影响[J]. 中国中西医结合杂志, 2003, 0(Z1) |
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| 作者姓名： | 章庆国 赵士芳 |
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| 作者单位： | 东南大学医学院整形外科 浙江大学医学院颌面外科 （南京 210009） （杭州310006） |
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| 摘 要： | 目的:研究积雪草甙(asiaticoside,Ad)和羟基积雪草甙(madecassoside,Md)对体外培养人成纤维细胞(human skin fibroblast,HSFb)的增殖及胶原蛋白合成的影响。方法:应用 XTT 方法比较研究 Ad 和Md 对 HSFb 增殖的影响,应用放射免疫的方法测定各实验组和空白对照组细胞上清液中Ⅰ型和Ⅲ型前胶原氨基端肽原(procollagen Ⅰ of aminoterminal propeptide,PINP;procollagen Ⅲ of aminoterminal propeptide,PⅢNP)的含量。结果:(1)Ad 和 Md 在浓度高达200μg/ml 时仍未见任何细胞毒性;(2)与空白对照组相比,Ad 和 Md 对 HSFb 有明显促进增殖作用(P<0.05);两者同时也可有效刺激 HSFb PⅠNP 和 PⅢNP 分泌;(3)Ad 和 Md 刺激靶细胞 PⅠNP 和 PⅢNP 分泌的生物学作用呈现明显的量效关系;(4)在一定剂量范围内,对促进 HSFb 增殖以及刺激 PⅠNP 分泌,Ad 显示出比 Md 更强的生物活性(P<0.05,P<0.01)。...
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| 关 键 词： | 积雪草甙 羟基积雪草甙 成纤维细胞 Ⅰ型前胶原氨基端肽原 Ⅲ型前胶原氨基端肽原 |
Effects of Asiaticoside and Madecassoside on Cells Proliferation and Synthesis of Collagen Type Ⅰ and Type Ⅲ in Cultured Human Skin Fibroblast |
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| ZHANG Qing-guo,ZHAO Shi-fang. Effects of Asiaticoside and Madecassoside on Cells Proliferation and Synthesis of Collagen Type Ⅰ and Type Ⅲ in Cultured Human Skin Fibroblast[J]. Chinese journal of integrated traditional and Western medicine, 2003, 0(Z1) |
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| Authors: | ZHANG Qing-guo ZHAO Shi-fang |
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| Affiliation: | Department of Maxillofacial Surgery; Medical College of Zhejiang University; Hangzhou(310006); |
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| Abstract: | Objective:To study the effects of asiaticoside (AD) and madecassoside (MD) on the cell proliferation and the type Ⅰ and type Ⅲ collagen synthesis in cultured human skin fibroblast (HSF) in vitro.Methods:The effect of AD and MD was determined on prolifera- tion of cultured fibroblast by XTT method,and the protein content of procollagen Ⅰ of aminoterminal propeptide (P Ⅰ NP) and procol- lagen Ⅲ of aminoterminal propeptide (P Ⅲ NP) in the culture medium of treated groups and control groups were measured by rad... |
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| Keywords: | asiaticoside madecassoside fibroblast procollagen I of aminoterminal propeptide procollagen Ⅲ of aminoterminal propeptide |
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