Evaluation of a whole‐blood chemiluminescent immunoassay of IFN‐γ, IP‐10, and MCP‐1 for diagnosis of active pulmonary tuberculosis and tuberculous pleurisy patients |
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| Authors: | Hang Li Yourong Yang Jianyang Liu Ting Yu Xueqiong Wu |
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| Affiliation: | 1. The Tumor Hospital of Jilin Province, Changchun, China;2. Army Tuberculosis Prevention and Control Key Laboratory, Beijing Key Laboratory of New Techniques of Tuberculosis Diagnosis and Treatment, Institute of Tuberculosis Research, the 309th Hospital of PLA, Beijing, China;3. The Second Hospital of Jilin University, Changchun, China |
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| Abstract: | The study explored the use of IP‐10, MCP‐1, and IFN‐γ as biomarkers to improve the diagnoses of active pulmonary tuberculosis and tuberculous pleurisy. We enrolled 267 individuals, including 134 TB patients, 93 patients with non‐tuberculous pulmonary diseases, and 40 healthy controls. Whole bloods were stimulated in vitro with rCFP‐10/ESAT‐6 protein antigen of Mycobacterium tuberculosis. The levels of IFN‐γ, IP‐10, and MCP‐1 in cultured supernatants of whole bloods were detected by a chemiluminescence immunoassay. A receiver operating characteristic (ROC) curve was drawn to determine the cutoff value for diagnosing TB and to evaluate the diagnostic efficacies of the IFN‐γ, IP‐10, and MCP‐1 for TB. The antigen‐specific release of each cytokine, IFN‐γ, IP‐10, and MCP‐1, was significantly higher in the TB groups than in either the non‐tuberculous pulmonary disease group (p < 0.001) or the healthy control group (p < 0.001). The ROC curves indicated cutoff values for IFN‐γ, IP‐10, and MCP‐1 at 147.8, 160.4, and 496.4 pg/mL, respectively. The sensitivity, specificity, PPV, NPV, and diagnostic efficiency for IFN‐γ were 85.8%, 70.7%, 74.7%, 83.2%, and 78.3%, respectively; for IP‐10 were 72.4%, 75.9%, 75.2%, 73.2%, and 74.2%, respectively; and for MCP‐1 were 90.3%, 97.0%, 96.8%, 90.8%, and 93.6%, respectively. IFN‐γ combined MCP‐1 improved the sensitivity to 97.8% compared with IFN‐γ (p < 0.001). Our findings indicate high sensitivity and specificity of MCP‐1 as novel biomarkers for the diagnosis of active pulmonary tuberculosis and tuberculous pleurisy. |
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| Keywords: | Tuberculosis diagnosis IFN‐γ IP‐10 MCP‐1 |
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