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| 骨髓干细胞移植可影响心肌梗死后心肌细胞的凋亡 | |
| 引用本文： | 张瑞成,董念国,侯剑峰,法宪恩. 骨髓干细胞移植可影响心肌梗死后心肌细胞的凋亡[J]. 心脏杂志, 2007, 19(4): 388-390,398. DOI: 10.13191/j.chj.2007.04.22.zhangrch.009 |
| 作者姓名： | 张瑞成 董念国 侯剑峰 法宪恩 |
| 作者单位： | 1. 华中科技大学附属协和医院心血管外科,湖北,武汉,430022;郑州大学第二附属医院心血管外科,河南,郑州,450014 2. 华中科技大学附属协和医院心血管外科,湖北,武汉,430022 3. 郑州大学第二附属医院心血管外科,河南,郑州,450014 |
| 摘 要： | 目的观察骨髓单个核细胞（bone marrowmononuclear cells,BM-MNCs）移植对大鼠急性心肌梗死（AMI）后心肌细胞凋亡的影响。方法健康雄性Wistar大鼠30只,随机均分为实验组和对照组。用结扎冠状动脉左前降支的方法建立大鼠AMI模型,将制备的BM-MNCs悬液和培养液分别经心外膜下植入实验组和对照组梗死心肌周围。移植术后4周末,检测心肌细胞凋亡情况及Bcl-2、Fas、FasL蛋白在心肌细胞中表达水平,并观察梗死区心肌内移植BM-MNCs及其周边区组织形态学特点。结果移植后4周末,实验组心肌细胞凋亡指数显著低于对照组（P<0.05）;与对照组相比,实验组Bcl-2蛋白吸光值显著升高（P<0.05）,Fas、FasL蛋白吸光值显著降低（P<0.05）;实验组心肌梗死区内有BrdU标记阳性的BM-MNCs存活。结论同种异体大鼠BM-MNCs移植可调节Bcl-2、Fas、FasL蛋白表达,抑制AMI后心肌细胞凋亡发生。 |
| 关 键 词： | 骨髓干细胞 细胞移植 心肌梗死 细胞凋亡 |
| 文章编号： | 1009-7236（2007）04-388-04 |
| 收稿时间： | 2006-07-28 |
| 修稿时间： | 2006-07-282006-10-10 |
Bone marrow mononuclear cells transplantation inhibits rat cardiac myocyte apoptosis after acute myocardial infarction |
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| ZHANG Rui-cheng,DONG Nian-guo,HOU Jian-feng,FA Xian-en. Bone marrow mononuclear cells transplantation inhibits rat cardiac myocyte apoptosis after acute myocardial infarction[J]. Chinese Heart Journal, 2007, 19(4): 388-390,398. DOI: 10.13191/j.chj.2007.04.22.zhangrch.009 | |
| Authors: | ZHANG Rui-cheng DONG Nian-guo HOU Jian-feng FA Xian-en |
| Affiliation: | 1. Department of Cardiovascular Surgery, Union Hospital Affiliated to Huazhong University of Science and Technique, Wuhan 430022, Hubei, China; 2. Department of Cardiovascular Surgery, Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan, China |
| Abstract: | AIM To observe the effects of allogeneic bone marrow mononuclear cells(BM-MNCs)on rat cardiac myocyte apoptosis after acute myocardial infaretion(AMI).METHODS Thirty male healthy Wistar rats were randomly divided into two groups: control group(n=15)and transplantation group(n=15).As soon as the descending anterior branch of left coronary artery was ligated,DMEM-LG medium was injected into the peri-infarct area of control group and BM-MNCs labeled with BrdU were injected into the peri-infarct area of transplantation group.Apoptotic cells and the expression of Bcl-2,Fas and FasL were detected 4 weeks later.RESULTS Four weeks after the transplantation,cardiomyocyte apoptotic index and the expression of Fas and FasL decreased significantly(P<0.05) while the expression of Bcl-2 increased significantly(P<0.05)in the transplantation group,compared with those in the control group.BM-MNCs labeled with BrdU were observed around the injected sites by immunohistochemical examination.CONCLUTION Allogeneic BM-MNCs transplantation regulate the expression of Bcl-2,Fas and FasL and inhibits cardiomyocyte apoptosis after AMI. |
| Keywords: | bone marrow mononuclear cells cell transplantation myocardial infarction apoptosis |
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