Association study of interleukin‐1 family and interleukin‐6 gene single nucleotide polymorphisms in recurrent aphthous stomatitis |
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| Authors: | S. Najafi H. Yousefi M. Mohammadzadeh A. Z. Bidoki I. Firouze Moqadam E. Farhadi A. A. Amirzargar N. Rezaei |
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| Affiliation: | 1. Dental Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran;2. Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran;3. Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran;4. Department of Oral Medicine, Dental Branch, Tehran Azad University, Tehran, Iran;5. Molecular Immunology Research Center;6. and Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;7. Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran;8. Hematology Department, School of Allied Medical Science, Iran University of Medical Sciences, Tehran, Iran;9. Universal Scientific Education and Research Network (USERN), Tehran, Iran |
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| Abstract: | Recurrent aphthous stomatitis (RAS) is a common painful, ulcerative oral inflammatory disorder with unknown aetiology. Immune system and aberrant cytokine cascade deemed to be critical in outbreaks of RAS ulcers. Interleukin‐1 (IL‐1) and IL‐6 are the most potent pro‐inflammatory cytokines. Single nucleotide polymorphisms (SNPs) of IL‐1 and IL‐6 genes can affect the secretion of these cytokines. The aim of this study was to investigate the association between RAS and IL‐6 and IL‐1 in Iranian subjects with minor RAS. Genomic DNA was obtained from 64 Iranian patients with RAS. IL‐1α C ?889 T, IL‐1β C ?511 T, IL‐1β C +3962 T, IL‐1R C pst‐I 1970 T, IL‐1Ra C Mspa‐I11100 T, IL‐6 C ?174 G and IL‐6 A nt +565 G polymorphisms were determined using polymerase chain reaction with sequence‐specific primers (PCR‐SSP). The frequency of C ?174 C genotype in the patients group was significantly different from the healthy control. No other significant differences were found in genotype and alleles frequencies between the two groups. These results indicate that certain SNPs of IL‐6 gene at position ?174 which located in promoter have association with predisposition of individuals to RAS. |
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