| Abstract: | In this study, we developed a novel and simple HPLC-DAD method for simultaneous qualitative and quantitative determination of five major components of Glycyrrhizae Radix et Rhizoma (GRR) in Xiaoer Zhike Tangjiang (XEZKTJ) with standardized reference extract (SRE). The five analytes (liquiritin apioside, liquiritin, isoliquiritin apioside, liquirigenin and glycyrrhizic acid) were well separated with good linearity, precision, stability and repeatability. The recovery rates ranged from 95.69% to 100.80%. The content of the five compounds in 34 batches of commercial XEZKTJ products was determined using standardized GRR extract (SRE method) and individual chemical reference standards (CRS method). Highly similar results were obtained from the two methods, demonstrating the feasibility of the proposed SRE method. Taken together, we proposed an efficient and low-cost way to perform multi-component quality control of XEZKTJ in this study. |
