| STIM1在骨肉瘤细胞增殖和转移中的作用及机制研究 |
| |
| 引用本文： | 宋大江,朱宝林. STIM1在骨肉瘤细胞增殖和转移中的作用及机制研究[J]. 现代肿瘤医学, 2021, 0(4): 569-573. DOI: 10.3969/j.issn.1672-4992.2021.04.006 |
| |
| 作者姓名： | 宋大江 朱宝林 |
| |
| 作者单位： | 徐州医科大学附属沭阳医院，江苏 沭阳 223600 |
| |
| 摘 要： | 目的:探究STIM1对骨肉瘤细胞143B增殖和转移的作用及其具体机制研究.方法:使用shRNA构建STIM1敲减的143B稳转细胞株;通过实时荧光定量PCR技术和Western Blot技术检测细胞敲减效率,通过克隆形成实验和CCK-8检测STIM1对骨肉瘤细胞143B增殖的影响;通过Transwell实验观察STIM...
|
| 关 键 词： | 骨肉瘤 STIM1 增殖 转移 |
The effect and mechanism of STIM1 in proliferation and metastasis of osteosarcoma cells |
| |
| SONG Dajiang,ZHU Baolin. The effect and mechanism of STIM1 in proliferation and metastasis of osteosarcoma cells[J]. Journal of Modern Oncology, 2021, 0(4): 569-573. DOI: 10.3969/j.issn.1672-4992.2021.04.006 |
| |
| Authors: | SONG Dajiang ZHU Baolin |
| |
| Affiliation: | Shuyang Hospital Affiliated to Xuzhou Medical University，Jiangsu Shuyang 223600,China. |
| |
| Abstract: | Objective:To investigate the effect of STIM1 on proliferation and metastasis of osteosarcoma 143B cells and its underlying mechanism.Methods:STIM1 was silenced in 143B cell line through shRNA.The expression of STIM1 was detected by Western Blot and real-time PCR,respectively.The effect of STIM1 on the proliferation of 143B was detected by colony formation assay and CCK-8.The effect of STIM1 on the migration and invasion of osteosarcoma cells was observed by Transwell assay.The protein levels of matrix metalloproteinases MMP-2 and MMP-9 were detected by Western Blot.Results:After STIM1 was silenced in osteosarcoma cells,the proliferation capacity of the cells decreased significantly.In addition,downregulation of STIM1 effectively inhibited the migration and invasion of osteosarcoma cells,and downregulated the expression protein levels of MMP-2 and MMP-9 in 143B cells.Conclusion:Knockdown of STIM1 can effectively downregulate the protein levels of MMP-2 and MMP-9,thereby inhibiting cell proliferation,migration and invasion of osteosarcoma,which may be a new therapeutic target for osteosarcoma. |
| |
| Keywords: | osteosarcoma STIM1 proliferation metastasis |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《现代肿瘤医学》浏览原始摘要信息 |
|
点击此处可从《现代肿瘤医学》下载免费的PDF全文 |
