| 星形胶质细胞条件培养液对tbOOH致PC12细胞凋亡的抑制作用 |
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| 引用本文： | 莫永炎,邢飞跃,张宝,陈瑗,姜勇. 星形胶质细胞条件培养液对tbOOH致PC12细胞凋亡的抑制作用[J]. 南方医科大学学报, 2004, 24(9): 998-1000 |
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| 作者姓名： | 莫永炎 邢飞跃 张宝 陈瑗 姜勇 |
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| 作者单位： | 1. 第一军医大学, 病理生理学教研室暨全军休克微循环重点实验室, 广东, 广州, 510515;2. 暨南大学生殖免疫研究中心, 广东, 广州, 510623;3. 第一军医大学, 自由基研究室, 广东, 广州, 510515 |
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| 基金项目： | 广东省自然科学基金(020016)~~ |
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| 摘 要： | 目的 探讨星形胶质细胞条件培养液对活性氧叔丁基脂氢过氧化物tbOOH诱导的PC12细胞凋亡的影响。方法 以分离、纯化的SD大鼠大脑皮层星形胶质细胞制备的条件培养液培养tbOOH应激的PC12细胞,采用荧光显微镜和透射电镜形态观察细胞凋亡;用流式细胞术测定细胞凋亡率的变化;用巴比妥酸法评估细胞内丙二醛含量的变化。结果 tbOOH可诱导PC12细胞凋亡,而星形胶质细胞条件培养液可降低其凋亡;同时,巴比妥酸法显示星形胶质细胞条件培养液可显著性降低PC12细胞丙二醛含量(P<0.05)。结论 星形胶质细胞条件培养液有提高PC12细胞抗氧化能力,可抑制活性氧tbOOH诱导的神经细胞凋亡。
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| 关 键 词： | 星形胶质细胞条件培养液/抗氧化作用 叔丁基脂氢过氧化物 PC12细胞/凋亡 活性氧种 |
| 文章编号： | 1000-2588(2004)09-0998-03 |
| 修稿时间： | 2003-05-21 |
Effect of astrocyte-conditioned medium on tert-butyl hydroperoxide-induced apoptosis of PC12 cells |
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| MO Yong-yan,XING Fei-yue,ZHANG Bao,CHEN Yuan,JIANG Yong. Effect of astrocyte-conditioned medium on tert-butyl hydroperoxide-induced apoptosis of PC12 cells[J]. Journal of Southern Medical University, 2004, 24(9): 998-1000 |
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| Authors: | MO Yong-yan XING Fei-yue ZHANG Bao CHEN Yuan JIANG Yong |
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| Affiliation: | MO Yong-yan1,XING Fei-yue2,ZHANG Bao3,CHEN Yuan3,JIANG Yong1Department of Pathophysiology and Key Lab of Shock and Microcirculation of PLA1,Laboratory of Free Radicals3,First Military Medical University,Guangzhou 510515,China, 2Center for Reproductive Immunology Research,Ji'nan University,Guangzhou 510632,China |
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| Abstract: | Objective To investigate the effect of astrocyte-conditioned medium (ACM) on PC12 cell apoptosis induced by reactive oxygen species tert-butyl hydroperoxide (tbOOH). Methods PC12 cells attacked by tbOOH were cultured with Sprague Dawley rat cerebral cortex ACM, and the cell apoptosis was observed by fluorescent microscopy and transmission electron microscopy. The cell apoptosis rate was evaluated by flow cytometry and malondialdehyde (MDA) concentration measured using thiobarbituric acid (TBA)-reacting substances (TRABS). Result TbOOH induced PC12 cell apoptosis, and ACM significantly decreased the apoptosis rate and MDA concentration in tbOOH-treated cells. Conclusion ACM may increase the anti-oxidation capacity of PC12 cells and inhibit cell apoptosis induced by tbOOH. |
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| Keywords: | astrocyte-conditioned medium/anti-oxidation tert-butyl hydroperoxide PC12 cells/cell apoptosis reactive oxidative species |
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