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| HPLC法测定人血浆中的泮托拉唑 | |
| 引用本文： | 徐海燕,杨丽,张鹏,申涛,赵怀清. HPLC法测定人血浆中的泮托拉唑[J]. 沈阳药科大学学报, 2008, 25(1): 44-47 |
| 作者姓名： | 徐海燕 杨丽 张鹏 申涛 赵怀清 |
| 作者单位： | 1. 沈阳药科大学,药学院,辽宁,沈阳,110016 2. 辽宁电力中心医院,辽宁,沈阳,110015 |
| 摘 要： | 目的建立测定人血浆中泮托拉唑的HPLC法。方法血浆样品经液-液萃取后,以甲醇-水(体积比为60∶40)为流动相,倍他米松为内标,采用Diamonsil C18柱(4.6 mm×150 mm,5μm)分离,在288 nm处进行检测。结果标准曲线线性范围为0.02~5.0 mg.L-1,定量下限为20μg.L-1,日内和日间精密度(RSD)均小于8.4%,准确度在104.3%以内,低、中、高3个质量浓度的提取回收率分别为75.4%、64.8%和65.3%。结论该法操作简便、快速、灵敏,适用于泮托拉唑的临床药物动力学及制剂的生物等效性评价。 |
| 关 键 词： | 泮托拉唑 高效液相色谱法 药物动力学 血药浓度 |
| 文章编号： | 1006-2858（2008）01-0044-04 |
| 收稿时间： | 2007-01-17 |
| 修稿时间： | 2007-01-17 |
Determination of pantoprazole in human plasma by HPLC |
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| XU Hai-yan,YANG Li,ZHANG Peng,SHEN Tao,ZHAO Huai-qing. Determination of pantoprazole in human plasma by HPLC[J]. Journal of Shenyang Pharmaceutical University, 2008, 25(1): 44-47 | |
| Authors: | XU Hai-yan YANG Li ZHANG Peng SHEN Tao ZHAO Huai-qing |
| Affiliation: | 1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Liaoning Electronic Center Hospital, Shenyang 110016, China |
| Abstract: | Objective To develop an HPLC method for the determination of pantoprazole in human plasma. Method After a simple liquid-liquid extraction, pantoprazole and the internal standard were separated on Diamonsil C18 column and detected at the wavelength of 288 nm. The mobile phase consisted of methanol-water (V∶V= 60∶40 ). Results The linear calibration curve was obtained in the concentration range of 0.02-5.0 mg·L-1. The lower limit of quantification was 20 μg·L-1. The intra- and inter-day precision (RSD) were less than 8.4%, and the accuracy was within 104.3% calculated from QC samples. The extraction recoveries of pantoprazole at different levels (low, medium, high) were 75.4%, 64.8% and 65.3%, respectively. Conclusion The method is proved to be suitable for the clinical investigation of pharmacokinetics of pantoprazole, which offers the advantage of simplicity, speed and sensitivity. |
| Keywords: | pantoprazole HPLC pharmacokinetics plasma concentration |
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