| 尖吻蝮蛇蛇毒纤溶酶的分离纯化及氨基酸序列测定 |
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| 引用本文： | 郑颖, 沈居仁, 范泉水, 邱薇. 尖吻蝮蛇蛇毒纤溶酶的分离纯化及氨基酸序列测定[J]. 中国药科大学学报, 2007, 38(5): 469-471. |
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| 作者姓名： | 郑颖 沈居仁 范泉水 邱薇 |
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| 作者单位： | 成都军区疾病预防控制中心军事医学研究所,昆明,650032 |
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| 摘 要： | 目的:从尖吻蝮蛇蛇毒中分离纯化出高纯度的纤溶酶并对其进行初步性质研究和氨基酸序列分析。方法:采用DEAE-Sepharose FF离子交换和两次Superdex75PG凝胶过滤,从尖吻蝮蛇蛇毒中纯化出可直接溶解纤维蛋白原的蛋白酶。结果:分离到的纤溶酶相对分子质量为25kD,HPLC分析纯度在97%以上,氨基酸序列分析其N末端为MSTEF QRYME IVVNHS MVKKY NGDSD KIKAW。结论:采用此工艺可从尖吻蛇毒中分离出高比活的纤溶酶,为进一步研究其药理作用奠定了基础。
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| 关 键 词： | 尖吻蝮蛇蛇毒 纤溶酶 纯化 氨基酸序列 |
| 文章编号： | 1000-5048（2007）05-0469-03 |
| 修稿时间： | 2007-05-14 |
Purification and determination amino acid sequence of fibrinolytic enzyme from Agkistrodon acutus venom |
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| ZHENG Ying,SHEN Ju-ren,FAN Quan-shui,QIU Wei. Purification and determination amino acid sequence of fibrinolytic enzyme from Agkistrodon acutus venom[J]. Journal of China Pharmaceutical University, 2007, 38(5): 469-471 |
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| Authors: | ZHENG Ying SHEN Ju-ren FAN Quan-shui QIU Wei |
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| Affiliation: | Institute of Military Medical, Center for Disease Control and Prevention of Chengdu Military Area, Kunming 650032, China |
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| Abstract: | Aim:To isolate the fibrinolytic enzyme from Agkistodon acutus venom,and define its characters and the amino acid sequence of the product.Methods:The fibrinolytic enzyme was purified from the Agkistrodon acutus venom through DEAE-Sepharose FF ion-exchange chromatography and Superdex 75 PG gel column chromatography.Results:The molecular weight of the separated enzyme was 25 kD by means of SDS-PAGE and its purity is more than 97% estimated by HPLC.The N-terminal sequence of the product was confirmed as MSTEF QRYME IVVNHS MVKKY NGDOD KIKAW.Conclusion:The process is practicable for separation of pure fibrinolytic enzyme with high specific activity from Agkistrodon acutus venom.It lay a foundation for the further pharmacological study. |
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| Keywords: | Agkistodon acutus venom fibrinolytic enzyme purification amino acid sequence |
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