| 椎间注射转化生长因子β1对兔退变腰椎间盘蛋白多糖表达的影响 |
| |
| 引用本文： | 吴 彬,张 辉,王海滨,贾存岭,赵益峰. 椎间注射转化生长因子β1对兔退变腰椎间盘蛋白多糖表达的影响[J]. 中国组织工程研究, 2011, 15(11): 1961-1964. DOI: 10.3969/j.issn.1673-8225.2011.11.015 |
| |
| 作者姓名： | 吴 彬 张 辉 王海滨 贾存岭 赵益峰 |
| |
| 作者单位： | 1济宁医学院附属医院骨外科,山东省济宁市 2720002 泰山医学院附属医院骨外科,山东省泰安市 271000 |
| |
| 摘 要： | 背景：体外研究证实转化生长因子β1可以促进蛋白多糖合成,延缓椎间盘退变,但体内实验鲜见报道。目的：观察局部应用转化生长因子β1对兔退变腰椎间盘髓核蛋白多糖表达的影响。方法：取30只新西兰大白兔建立兔腰椎间盘退变模型,造模12周,经X射线证实退变后,随机选择6只模型兔及6只未造模正常兔,处死取材。分别向剩余24只模型兔L4~5椎间隙注射转化生子因子β1和生理盐水。末次给药2,4周取材,间苯三酚法测定兔椎间盘髓核内蛋白多糖的水平。结果与结论：造模12周,兔退变椎间盘髓核中蛋白多糖水平明显降低(P < 0.01)。经局部注射转化生子因子β1后,兔退变椎间盘髓核中蛋白多糖表达明显增多(P < 0.01)。说明在兔椎间注射转化生子因子β1 可以促进髓核蛋白多糖的合成,延缓椎间盘退变。
|
| 关 键 词： | 椎间盘退变 蛋白多糖 转化生长因子&beta 1 兔 动物模型 |
| 收稿时间： | 2010-09-28 |
Effects of intervertebral injection of transforming growth factor beta 1 on proteoglycan expression in rabbit degenerated lumbar discs |
| |
| Wu Bin,Zhang Hui,Wang Hai-bin,Jia Cun-ling,Zhao Yi-feng. Effects of intervertebral injection of transforming growth factor beta 1 on proteoglycan expression in rabbit degenerated lumbar discs[J]. Chinese Journal of Tissue Engineering Research, 2011, 15(11): 1961-1964. DOI: 10.3969/j.issn.1673-8225.2011.11.015 |
| |
| Authors: | Wu Bin Zhang Hui Wang Hai-bin Jia Cun-ling Zhao Yi-feng |
| |
| Affiliation: | 1Department of Orthopedics, Affiliated Hospital of Jining Medical College, Jining 272000, Shandong Province, China
2 Department of Orthopedics, Affiliated Hospital of Taishan Medical College, Taian 271000, Shandong Province, China |
| |
| Abstract: | BACKGROUND:In vitro experiments have confirmed that transforming growth factor-β1 (TGF-β1) can promote proteoglycan synthesis and delay intervertebral disc degeneration, but its in vivo role remains unclear. OBJECTIVE:To study the effect of local utilization of TGF-β1 on proteoglycan expression in rabbit degenerated lumbar discs.METHODS:Thirty-six New Zealand white rabbits were divided into two groups randomly, with 6 in control group and 30 in model group. The lumbar structure was destroyed partly in the model group. After 12 weeks, the intervertebral disc degeneration was testified by X-ray in model group. Six rabbits chosen randomly from the model group and all rabbits in the control group were killed and the organizations of the intervertebral discs of L4-5 were harvested. Meanwhile, the rest rabbits in model group were divided into two groups randomly, which was injected with TGF-β1 saline. The rabbits were killed by 2 and 4 weeks after injection. The contents of proteoglycan in nucleus pulposus were tested by phloroglucinol method. RESULTS AND CONCLUSION:The content of proteoglycan in the nucleus pulposus was obviously decreased at 12 weeks after model preparation (P < 0.01). After TGF-β1 injection, the content of proteoglycan was notably increased (P < 0.01). It is suggested that intervertebral injection of TGF-β1 to rabbit can delay intervertebral disc degeneration and enhance the synthesis of proteoglycan in nucleus pulposus. |
| |
| Keywords: | |
|
| 点击此处可从《中国组织工程研究》浏览原始摘要信息 |
|
点击此处可从《中国组织工程研究》下载全文 |
|
