| 转化生长因子β1在兔房水中的浓度变化 |
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| 引用本文： | 吴 伟,廖国平,陆晓和,梁丽芳,闫丽梦,陈 晗,张海峰,徐 微,魏二霞. 转化生长因子β1在兔房水中的浓度变化[J]. 中国组织工程研究, 2011, 15(37): 6871-6874. DOI: 10.3969/j.issn.1673-8225.2011.37.008 |
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| 作者姓名： | 吴 伟 廖国平 陆晓和 梁丽芳 闫丽梦 陈 晗 张海峰 徐 微 魏二霞 |
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| 作者单位： | 南方医科大学珠江医院, 眼科, 药剂科,广东省广州市 510282 |
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| 摘 要： | 背景：转化生长因子β1可参与角膜损伤后的修复。目的：观察转化生长因子β1滴眼液滴眼后房水中的浓度变化规律。方法：将新西兰大白兔随机分为5组,分别给予PBS和质量浓度为0.5,1.0,2.0,4.0 mg/L的转化生长因子β1滴眼液滴右眼。结果与结论：通过裂隙灯观察兔角膜和结膜结构,各组兔眼均无结膜分泌物、球结膜充血、角膜水肿增厚、角膜后沉着物、前房炎性反应及晶状体混浊改变。ELISA检测结果显示,与PBS组比较,质量浓度2.0和4.0 mg/L转化生长因子β1滴眼液能有效提高兔眼房水中转化生长因子β1的质量浓度(P < 0.01),角膜穿透性良好,在房水中可以达到有效的治疗浓度。
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| 关 键 词： | 转化生长因子&beta 1 房水 角膜 兔 组织构建 |
| 收稿时间： | 2011-04-23 |
Concentrations of transforming growth factor beta 1 in rabbit aqueous humor |
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| Wu Wei,Liao Guo-ping,Lu Xiao-he,Liang Li-fang,Yan Li-meng,Chen Han,Zhang Hai-feng,Xu Wei,Wei Er-xia. Concentrations of transforming growth factor beta 1 in rabbit aqueous humor[J]. Chinese Journal of Tissue Engineering Research, 2011, 15(37): 6871-6874. DOI: 10.3969/j.issn.1673-8225.2011.37.008 |
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| Authors: | Wu Wei Liao Guo-ping Lu Xiao-he Liang Li-fang Yan Li-meng Chen Han Zhang Hai-feng Xu Wei Wei Er-xia |
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| Affiliation: | Department of Ophthalmology,Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China |
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| Abstract: | BACKGROUND:Studies has demonstrated that transforming growth factor β1(TGF-β1) eyedrops is not only involved in the tissue metabolism of normal eye as well as kinds of physiological function, but also plays an important role in the treatment effect on corneal wound healing, uveitis and nearsightedness .OBJECTIVE:To investigate the concentration of TGF-β1 in aqueous humor after ophthalmic administration and to establish the method for quantitatively analyzing the TGF-β1 levels in rabbit aqueous humor.METHODS:Thirty rabbits were randomly divided into blank control, TGF-β1 0.5, 1.0, 2.0, 4.0 mg/L groups, with six animals in each group. PBS and 0.5, 1.0, 2.0,4.0 mg/L TGF-β1 were dropped into the right eyeballs four times per day for 1 week. RESULTS AND CONCLUSION:No conjunctival secretion, bulbar conjunctiva hyperemia, corneal edema, keratic precipitates, inflammation in anterior chamber, or vitreous haze lens opacity was found prior to and following experiments in each group. ELISA results showed that compared with PBS, both 2.0 mg/L and 4.0 mg/L TGF-β1 eyedrops can significantly improve the TGF-β1 concentration effectively in rabbit aqueous humor (P < 0.05), which has a good penetrating ability in the cornea, and can effectively enhance treatment concentration in aqueous humor. |
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