| HPLC-DAD-ELSD法同时测定小柴胡汤正丁醇有效部位中7种成分的含量(英文) |
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| 引用本文： | 原红霞,李慧峰,裴妙荣. HPLC-DAD-ELSD法同时测定小柴胡汤正丁醇有效部位中7种成分的含量(英文)[J]. 中国药学, 2015, 24(4): 236-240. DOI: 10.5246/jcps.2015.04.031 |
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| 作者姓名： | 原红霞 李慧峰 裴妙荣 |
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| 作者单位： | 山西中医学院;山西医科大学 |
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| 摘 要： | 本文建立了同时测定小柴胡汤正丁醇有效部位中甘草苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素、人参皂苷Re和人参皂苷Rb1含量的HPLC-DAD-ELSD法。采用Diamonsil C18(2)色谱柱(4.6 mm×250 mm,5.0μm),以乙腈–0.05%甲酸水溶液为流动相,梯度洗脱,流速1 mL/min,二极管阵列检测器的检测波长为276 nm,蒸发光散射检测器的漂移管温度为110 oC,载气流速氮气2.1 L/min,柱温为30 oC。结果表明:7种有效成分在一定范围内线性关系良好。该方法简便、快速、重复性好,可用于同时测定小柴胡汤正丁醇有效部位中7种有效成分的含量。
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| 关 键 词： | HPLC-DAD-ELSD 正丁醇有效部位 小柴胡汤 同时测定 |
| 收稿时间： | 2015-01-14 |
Simultaneous determination of seven active components in n-butanol effective fraction of Xiao Chai Hu Tang by HPLC-DAD-ELSD |
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| Hongxia Yuan,Huifeng Li,Miaorong Pei. Simultaneous determination of seven active components in n-butanol effective fraction of Xiao Chai Hu Tang by HPLC-DAD-ELSD[J]. Journal of Chinese Pharmaceutical Sciences, 2015, 24(4): 236-240. DOI: 10.5246/jcps.2015.04.031 |
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| Authors: | Hongxia Yuan Huifeng Li Miaorong Pei |
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| Abstract: | An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components (liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1) in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18(2) column (4.6 mm × 250 mm, 5 μm) was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid (0.05%, v/v). Gradient elution was carried out at the flow rate of 1 mL/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang. |
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| Keywords: | HPLC-DAD-ELSD n-Butanol effective fraction Xiao Chai Hu Tang Simultaneous determination |
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